Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2598175	M	M	NA	Agree	0.00	1.59	0.00	11.82	3.54	44.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.960332	0.016337	0.01817	0.00516	92.55	2.59	4.73	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598176	M	M	NA	Agree	0.00	1.27	0.00	12.68	4.03	46.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.954185	0.020314	0.020368	0.005134	91.83	2.62	5.41	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598177	M	M	NA	Agree	0.00	2.18	0.00	12.43	3.65	42.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.957196	0.018357	0.020062	0.004385	92.15	2.76	4.92	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598178	M	M	NA	Agree	0.00	1.74	0.00	11.11	3.73	43.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.959369	0.016963	0.018858	0.004809	91.37	2.72	5.76	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598179	M	M	NA	Agree	0.00	5.44	0.00	37.18	7.02	67.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.94672	0.026364	0.020594	0.006321	90.57	2.57	6.69	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598180	M	M	NA	Agree	0.02	5.50	0.00	39.56	5.44	61.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.95221	0.023354	0.019299	0.005138	92.02	2.54	5.27	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598181	M	M	NA	Agree	0.00	4.39	0.00	30.77	6.08	55.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950503	0.024632	0.02031	0.004556	92.31	2.66	4.83	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598182	M	M	NA	Agree	0.00	4.96	0.00	37.03	6.82	60.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950051	0.02431	0.020232	0.005407	92.52	2.59	4.73	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598183	M	M	NA	Agree	0.02	4.70	0.00	37.37	6.36	61.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950379	0.023658	0.01976	0.006203	92.84	2.55	4.44	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598184	M	M	NA	Agree	0.01	5.69	0.00	32.27	5.69	62.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950933	0.022243	0.019758	0.007066	91.05	2.70	6.09	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598185	M	M	NA	Agree	0.00	5.64	0.00	39.30	6.44	67.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.953169	0.021581	0.019786	0.005465	91.54	2.47	5.82	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598186	M	M	NA	Agree	0.00	6.15	0.00	38.64	6.36	64.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950668	0.022826	0.020005	0.006501	92.44	2.73	4.63	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598187	M	M	NA	Agree	0.03	16.21	0.00	131.13	13.66	165.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91259	0.060502	0.02494	0.001968	91.28	1.93	6.59	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598188	M	M	NA	Agree	0.05	12.30	0.00	84.15	11.97	108.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.914102	0.059309	0.024492	0.002097	90.74	1.93	7.13	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598189	M	M	NA	Agree	0.00	11.97	0.00	81.18	12.83	133.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925103	0.047398	0.025201	0.002298	91.97	2.18	5.63	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2598190	M	M	NA	Agree	0.01	20.25	0.00	126.98	15.54	207.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923292	0.049708	0.025673	0.001327	91.20	1.92	6.70	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does