Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2449152	F	F	NA	Agree	34.83	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.84832	0.061323	0.040161	0.050196	90.99	6.74	2.03	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449153	F	F	NA	Agree	13.13	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.785688	0.056046	0.040382	0.117884	84.07	13.51	2.16	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449154	F	F	NA	Agree	28.52	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.82529	0.063102	0.039613	0.071996	88.49	9.08	2.17	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449155	F	F	NA	Agree	73.97	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.878502	0.06497	0.037395	0.019134	93.50	4.16	2.09	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449156	F	F	NA	Agree	110.80	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.874879	0.080006	0.037148	0.007966	94.64	2.90	2.20	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449157	F	F	NA	Agree	143.88	0.00	0.00	0.00	0.02	0.05	NONE	POLYA	0.827333	0.100075	0.038105	0.034488	92.53	5.33	1.86	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449158	F	F	NA	Agree	115.60	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.873337	0.081332	0.037902	0.007429	94.50	2.82	2.41	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449159	F	F	NA	Agree	98.79	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.874097	0.075235	0.038111	0.012557	94.04	3.40	2.29	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449160	F	F	NA	Agree	79.83	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.881395	0.072889	0.037649	0.008066	94.30	3.21	2.24	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449161	F	F	NA	Agree	111.87	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.873591	0.082496	0.040581	0.003332	94.68	2.50	2.55	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM2449162	F	F	NA	Agree	91.33	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.88209	0.071741	0.039239	0.00693	94.83	2.96	1.96	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does