Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM2069484	M	M	NA	Agree	0.01	2.82	0.00	14.29	3.15	18.76	NONE	RRNA_DEPLETED	0.679726	0.18837	0.121677	0.010227	76.72	14.53	3.88	5329	LP2 (2026-09-30)	PASS	NonPolyA_perM 5329 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069485	M	M	NA	Agree	0.00	2.95	0.00	12.79	2.80	19.89	NONE	RRNA_DEPLETED	0.664622	0.202971	0.117013	0.015394	76.57	14.85	4.00	5440	LP2 (2026-09-30)	PASS	NonPolyA_perM 5440 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069486	M	M	NA	Agree	0.00	3.23	0.00	18.62	3.55	24.36	NONE	RRNA_DEPLETED	0.700477	0.170459	0.11795	0.011115	75.50	14.13	6.62	4442	LP2 (2026-09-30)	PASS	NonPolyA_perM 4442 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069487	M	M	NA	Agree	0.00	3.06	0.00	18.22	3.29	23.35	NONE	RRNA_DEPLETED	0.65645	0.206039	0.118792	0.018719	75.24	14.23	7.07	4630	LP2 (2026-09-30)	PASS	NonPolyA_perM 4630 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069488	M	M	NA	Agree	0.00	2.64	0.00	13.46	2.67	18.44	NONE	RRNA_DEPLETED	0.673517	0.187249	0.124315	0.014919	75.84	15.67	3.61	5177	LP2 (2026-09-30)	PASS	NonPolyA_perM 5177 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069489	M	M	NA	Agree	0.00	2.46	0.00	11.86	2.15	17.07	NONE	RRNA_DEPLETED	0.669627	0.173314	0.141998	0.01506	72.61	18.54	3.93	5882	LP2 (2026-09-30)	PASS	NonPolyA_perM 5882 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069490	M	M	NA	Agree	0.01	3.69	0.00	15.99	3.69	21.29	NONE	RRNA_DEPLETED	0.643683	0.236783	0.110818	0.008716	78.94	12.24	3.92	5253	LP2 (2026-09-30)	PASS	NonPolyA_perM 5253 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069491	M	M	NA	Agree	0.02	4.18	0.00	16.37	4.45	22.98	NONE	RRNA_DEPLETED	0.583089	0.298343	0.103858	0.014711	82.09	11.31	3.25	3334	LP2 (2026-09-30)	PASS	NonPolyA_perM 3334 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069492	M	M	NA	Agree	0.01	3.78	0.00	17.40	3.97	21.20	NONE	RRNA_DEPLETED	0.653998	0.224413	0.115239	0.006351	77.84	13.16	4.27	5801	LP2 (2026-09-30)	PASS	NonPolyA_perM 5801 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM2069493	M	M	NA	Agree	0.01	3.39	0.00	18.71	2.90	21.76	NONE	RRNA_DEPLETED	0.661569	0.207193	0.120462	0.010776	77.10	13.22	4.68	5251	LP2 (2026-09-30)	PASS	NonPolyA_perM 5251 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not