Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1585453	M	M	NA	Agree	0.00	5.55	0.00	32.46	15.71	72.47	NONE	POLYA	0.87683	0.043403	0.044628	0.035139	92.32	5.45	2.00	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585454	M	M	NA	Agree	0.01	4.98	0.00	30.20	15.02	66.79	NONE	POLYA	0.876191	0.047798	0.04555	0.030461	92.30	4.99	2.48	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585455	M	M	NA	Agree	0.00	5.79	0.00	35.69	16.15	67.58	NONE	POLYA	0.887787	0.037345	0.046259	0.02861	92.94	4.65	2.22	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585456	M	M	NA	Agree	0.00	6.45	0.00	37.19	14.00	68.52	NONE	POLYA	0.89065	0.035482	0.046937	0.026931	93.05	4.57	2.17	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585457	M	M	NA	Agree	0.00	5.84	0.00	29.61	13.24	72.96	NONE	POLYA	0.901757	0.030593	0.04536	0.02229	93.46	4.03	2.32	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585458	M	M	NA	Agree	0.00	7.53	0.00	32.36	15.33	59.09	NONE	POLYA	0.873306	0.053951	0.041542	0.031201	93.24	4.92	1.60	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585459	M	M	NA	Agree	0.00	7.03	0.00	35.57	16.03	79.98	NONE	POLYA	0.875725	0.044817	0.046339	0.033119	92.37	5.21	2.20	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585460	M	M	NA	Agree	0.00	5.58	0.00	31.81	16.65	71.28	NONE	POLYA	0.879242	0.047639	0.043698	0.029421	92.95	4.71	2.13	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585461	M	M	NA	Agree	0.00	6.30	0.00	38.82	15.59	77.53	NONE	POLYA	0.88951	0.041391	0.047055	0.022044	93.52	4.08	2.19	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1585462	M	M	NA	Agree	0.00	6.91	0.00	34.57	16.67	80.61	NONE	POLYA	0.895786	0.03341	0.047308	0.023496	93.32	4.22	2.26	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does