Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1581146	M	M	NA	Agree	0.00	4.31	0.00	32.00	18.92	47.07	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.89983	0.039397	0.052592	0.008182	71.58	2.68	25.52	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581147	M	M	NA	Agree	0.02	11.80	0.00	60.74	30.23	96.83	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.88313	0.052514	0.062133	0.002223	67.25	2.02	30.48	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581148	M	M	NA	Agree	0.02	4.40	0.00	32.93	18.95	53.32	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.903539	0.037813	0.052619	0.006028	75.59	2.54	21.66	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581149	M	M	NA	Agree	0.06	2.73	0.00	23.10	14.37	40.85	FIRST_READ_TRANSCRIPTION_STRAND	POLYA	0.907561	0.031964	0.051472	0.009003	74.49	2.98	22.31	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581153	M	M	NA	Agree	0.01	3.17	0.00	14.00	10.67	25.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907738	0.040773	0.041368	0.010121	95.84	3.54	0.40	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581154	M	M	NA	Agree	0.00	3.33	0.00	13.04	9.22	22.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913586	0.038265	0.040079	0.008071	96.13	3.18	0.45	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581155	M	M	NA	Agree	0.01	2.56	0.00	11.03	10.59	24.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912981	0.040105	0.039646	0.007269	96.28	3.09	0.40	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1581156	M	M	NA	Agree	0.01	2.75	0.00	11.55	7.77	28.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909427	0.040693	0.040904	0.008976	96.09	3.26	0.43	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does