Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM1133704	F	F	NA	Agree	167.74	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.913604	0.060813	0.02359	0.001993	87.17	3.12	9.52	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133705	F	F	NA	Agree	137.39	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.915784	0.057867	0.022428	0.003921	88.03	3.39	8.37	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133706	F	F	NA	Agree	51.70	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.902481	0.04109	0.020701	0.035728	84.68	5.89	9.28	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133707	F	F	NA	Agree	183.14	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909219	0.065236	0.023537	0.002007	85.72	2.99	11.09	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133708	F	F	NA	Agree	164.86	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.91442	0.060065	0.023232	0.002284	90.57	3.27	5.97	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133709	F	F	NA	Agree	94.74	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.925656	0.05049	0.021894	0.001959	90.37	3.17	6.30	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133710	F	F	NA	Agree	155.89	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.829065	0.057554	0.020994	0.092388	82.95	11.14	5.72	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133711	F	F	NA	Agree	154.50	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.914146	0.061031	0.022463	0.00236	90.19	3.26	6.36	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133712	F	Undetermined	NA	Conflict	0.24	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.91421	0.060057	0.022992	0.002741	85.85	3.33	10.63	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133713	F	F	NA	Agree	157.56	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.909296	0.06372	0.024554	0.00243	90.75	3.65	5.38	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133714	F	F	NA	Agree	159.03	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.913409	0.061156	0.02239	0.003045	87.38	3.20	9.23	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM1133715	F	F	NA	Agree	177.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.907797	0.066197	0.024204	0.001803	90.47	3.40	5.93	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does