Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9634308	M	M	NA	Agree	0.02	4.93	0.00	25.61	6.31	43.05	NONE	POLYA	0.923463	0.047239	0.023807	0.00549	93.57	2.10	4.17	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9634309	M	M	NA	Agree	0.01	6.61	0.00	29.31	6.26	54.35	NONE	POLYA	0.915636	0.050414	0.022902	0.011047	94.00	2.60	3.23	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9634310	M	M	NA	Agree	0.00	4.75	0.00	26.35	6.63	38.99	NONE	POLYA	0.922384	0.042066	0.02262	0.01293	94.44	2.72	2.70	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9634311	M	M	NA	Agree	0.01	4.78	0.00	18.60	6.39	41.16	NONE	POLYA	0.917766	0.048091	0.022993	0.011151	94.60	2.59	2.64	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9634312	M	M	NA	Agree	0.03	3.79	0.00	19.49	6.29	42.78	NONE	POLYA	0.92067	0.040929	0.022175	0.016226	94.05	3.06	2.75	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9634313	M	M	NA	Agree	0.02	4.94	0.00	22.73	9.32	45.83	NONE	POLYA	0.914625	0.046673	0.023834	0.014868	94.04	2.87	2.95	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does