Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9415174	M	M	NA	Agree	0.00	4.75	0.00	19.57	15.12	43.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917527	0.044834	0.032915	0.004724	91.13	1.98	6.76	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415175	M	M	NA	Agree	0.00	8.21	0.00	25.13	19.64	48.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906524	0.053392	0.034463	0.005621	91.28	2.12	6.45	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415176	M	M	NA	Agree	0.03	6.63	0.00	21.80	17.26	38.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91057	0.051679	0.032855	0.004895	91.13	1.95	6.78	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415177	M	M	NA	Agree	0.00	1.05	0.00	5.61	4.21	30.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931146	0.023757	0.034647	0.010451	89.20	3.33	7.30	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415178	M	M	NA	Agree	0.01	2.38	0.00	12.89	5.90	38.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924733	0.028729	0.03772	0.008818	89.31	2.88	7.64	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415179	M	M	NA	Agree	0.00	5.41	0.00	16.45	13.38	41.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91386	0.04675	0.035359	0.004032	91.78	1.97	6.10	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415180	M	M	NA	Agree	0.00	1.45	0.00	4.76	4.21	26.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929367	0.026949	0.034133	0.009551	88.98	3.03	7.78	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415181	M	M	NA	Agree	0.03	0.97	0.00	5.04	1.30	9.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853757	0.022994	0.040738	0.08251	77.06	10.68	12.07	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415182	M	M	NA	Agree	0.01	3.72	0.00	15.96	11.91	44.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921671	0.038027	0.034088	0.006214	89.44	2.33	8.08	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415183	M	M	NA	Agree	0.02	4.01	0.00	17.78	12.21	42.68	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921479	0.042142	0.032239	0.00414	91.42	1.91	6.51	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415184	M	M	NA	Agree	0.00	5.14	0.00	17.41	16.66	44.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90999	0.050055	0.034484	0.005472	90.53	2.09	7.24	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9415185	M	M	NA	Agree	0.00	5.75	0.00	21.41	17.10	45.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904509	0.05508	0.034605	0.005806	86.57	2.10	11.18	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does