Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-19) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9362923	M	M	NA	Agree	0.03	6.22	0.00	32.74	15.01	56.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873677	0.062559	0.043859	0.019904	65.91	2.77	31.18	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362924	M	M	NA	Agree	0.03	8.41	0.00	47.14	20.09	73.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873587	0.067572	0.044289	0.014551	65.07	2.17	32.61	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362925	M	M	NA	Agree	0.00	6.59	0.00	32.69	16.17	82.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87557	0.067061	0.045236	0.012133	61.44	2.29	36.12	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362926	M	M	NA	Agree	0.00	7.04	0.00	24.63	15.16	71.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878665	0.067002	0.040614	0.013718	73.50	2.99	23.33	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362927	M	M	NA	Agree	0.02	3.54	0.00	17.84	11.95	79.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889205	0.052847	0.044125	0.013823	56.64	2.43	40.78	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362928	M	M	NA	Agree	0.04	4.36	0.00	16.75	9.58	64.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904236	0.040678	0.043302	0.011785	56.79	2.53	40.54	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362929	M	M	NA	Agree	0.00	3.01	0.00	9.86	7.23	57.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8943	0.047091	0.045397	0.013211	52.45	2.57	44.82	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362930	M	M	NA	Agree	0.00	2.78	0.00	13.22	6.92	55.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89764	0.046548	0.043312	0.012499	51.33	2.66	45.86	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362931	M	M	NA	Agree	0.01	5.79	0.00	32.91	16.23	97.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89727	0.050174	0.041947	0.010609	63.58	2.34	33.93	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9362933	M	M	NA	Agree	0.00	6.34	0.00	22.72	12.38	75.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876059	0.065792	0.042851	0.015298	53.54	2.40	43.92	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does