Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 1 sample processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9237362	M	M	NA	Agree	0.01	1.90	0.00	19.76	6.27	39.35	NONE	POLYA	0.889082	0.058577	0.025209	0.027133	90.45	4.77	4.38	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237363	M	M	NA	Agree	0.05	0.27	0.00	1.37	0.79	5.56	NONE	POLYA	0.693423	0.173559	0.046785	0.086233	73.23	9.35	16.75	193	LP2 (2026-09-30)	PASS	NonPolyA_perM 193 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237364	M	M	NA	Agree	0.03	2.64	0.00	14.92	5.55	31.44	NONE	POLYA	0.860163	0.067923	0.025205	0.04671	87.92	7.60	4.12	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237365	M	M	NA	Agree	0.05	0.20	0.00	1.07	0.55	4.14	NONE	POLYA	0.788113	0.083064	0.026052	0.102771	74.71	16.89	7.76	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237366	M	M	NA	Agree	0.00	1.92	0.00	23.91	3.89	36.33	NONE	POLYA	0.918992	0.035725	0.020462	0.02482	91.18	4.35	4.07	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237367	M	M	NA	Agree	0.03	1.56	0.00	14.08	5.84	33.92	NONE	POLYA	0.873765	0.07419	0.031106	0.020939	89.42	4.37	5.68	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237368	M	M	NA	Agree	0.00	3.13	0.00	20.53	6.81	49.16	NONE	POLYA	0.904555	0.053824	0.026188	0.015433	91.04	3.90	4.69	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237369	M	M	NA	Agree	0.01	2.36	0.00	15.53	6.11	41.01	NONE	POLYA	0.878794	0.070994	0.029785	0.020427	89.54	4.42	5.63	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237370	M	M	NA	Agree	0.00	0.47	0.00	3.19	1.33	16.01	NONE	POLYA	0.856221	0.069232	0.025452	0.049095	84.99	6.83	7.77	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237371	M	M	NA	Agree	0.00	3.01	0.00	20.29	7.74	45.80	NONE	POLYA	0.865626	0.077251	0.031583	0.02554	90.10	4.67	4.86	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237372	M	M	NA	Agree	0.01	2.03	0.00	11.96	5.87	39.69	NONE	POLYA	0.870868	0.064662	0.027643	0.036826	88.80	5.58	5.26	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237373	M	M	NA	Agree	0.01	2.70	0.00	15.85	7.08	47.42	NONE	POLYA	0.848824	0.077463	0.030987	0.042726	87.70	6.20	5.66	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237374	M	M	NA	Agree	0.00	0.96	0.00	14.74	4.38	29.90	NONE	POLYA	0.883284	0.049527	0.020685	0.046504	88.69	6.44	4.45	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237375	M	M	NA	Agree	0.04	2.51	0.00	18.78	8.09	43.99	NONE	POLYA	0.84618	0.086773	0.0335	0.033547	89.40	5.56	4.62	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237376	M	M	NA	Agree	0.00	2.36	0.00	15.68	5.52	43.42	NONE	POLYA	0.8665	0.062469	0.026673	0.044358	89.04	6.57	4.08	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237377	M	M	NA	Agree	0.00	2.16	0.00	13.07	5.58	44.22	NONE	POLYA	0.868334	0.074452	0.031083	0.026131	88.98	5.01	5.56	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237378	M	M	NA	Agree	0.01	2.06	0.00	17.73	5.15	39.14	NONE	POLYA	0.907802	0.042574	0.022888	0.026736	90.23	4.69	4.70	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237379	M	M	NA	Agree	0.01	1.97	0.00	14.92	5.68	45.29	NONE	POLYA	0.889288	0.060695	0.025999	0.024019	90.64	4.42	4.59	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237380	M	M	NA	Agree	0.05	2.96	0.00	19.49	6.17	42.37	NONE	POLYA	0.854405	0.064557	0.023823	0.057214	87.97	7.34	4.37	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237381	M	M	NA	Agree	0.02	2.96	0.00	14.96	5.81	38.54	NONE	POLYA	0.854694	0.068531	0.023197	0.053578	88.32	7.53	3.85	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237382	M	M	NA	Agree	0.00	1.74	0.00	21.58	4.68	28.78	NONE	POLYA	0.9184	0.038144	0.021374	0.022082	88.48	4.34	6.63	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237383	M	M	NA	Agree	0.00	1.81	0.00	18.20	5.54	40.48	NONE	POLYA	0.903443	0.046935	0.024257	0.025366	90.79	4.60	4.20	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237384	M	M	NA	Agree	0.00	3.07	0.00	22.38	6.01	49.04	NONE	POLYA	0.913111	0.041245	0.022076	0.023568	90.64	4.49	4.50	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237385	M	M	NA	Agree	0.01	2.84	0.00	23.64	6.60	54.47	NONE	POLYA	0.894591	0.056509	0.026611	0.02229	90.87	4.43	4.34	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237386	M	M	NA	Agree	0.00	2.23	0.00	19.74	5.05	37.09	NONE	POLYA	0.881557	0.051074	0.024876	0.042492	89.37	6.09	4.18	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237387	M	M	NA	Agree	0.00	2.48	0.00	18.08	7.70	47.42	NONE	POLYA	0.864176	0.07294	0.03122	0.031665	89.28	5.25	5.09	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237388	M	M	NA	Agree	0.01	2.56	0.00	16.19	5.69	40.29	NONE	POLYA	0.871034	0.056507	0.024796	0.047662	88.31	6.62	4.72	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237389	M	M	NA	Agree	0.02	2.29	0.00	14.83	6.65	41.61	NONE	POLYA	0.816687	0.087976	0.030623	0.064714	86.07	8.41	5.07	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237390	M	M	NA	Agree	0.00	1.86	0.00	26.84	4.65	39.71	NONE	POLYA	0.910235	0.036935	0.020949	0.031881	90.05	4.81	4.71	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237391	M	M	NA	Agree	0.05	0.36	0.00	3.19	1.97	16.70	NONE	POLYA	0.846102	0.095477	0.03645	0.021971	78.60	4.58	15.86	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237392	M	M	NA	Agree	0.00	2.95	0.00	20.17	6.00	47.47	NONE	POLYA	0.899386	0.050384	0.024864	0.025366	90.06	4.61	4.98	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237393	M	M	NA	Agree	0.01	2.25	0.00	15.47	6.47	44.78	NONE	POLYA	0.853263	0.074062	0.03227	0.040405	87.35	6.19	6.04	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237394	M	M	NA	Agree	0.02	1.77	0.00	16.65	5.35	37.35	NONE	POLYA	0.892465	0.05798	0.025788	0.023766	89.11	4.38	6.04	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237395	M	M	NA	Agree	0.01	3.47	0.00	25.69	8.04	49.64	NONE	POLYA	0.881549	0.069088	0.02845	0.020913	91.08	4.07	4.54	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237396	M	M	NA	Agree	0.03	2.59	0.00	17.62	5.47	37.77	NONE	POLYA	0.849403	0.073487	0.026204	0.050905	88.53	6.80	4.34	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237397	M	M	NA	Agree	0.02	3.53	0.00	21.08	6.43	39.03	NONE	POLYA	0.839724	0.072178	0.023385	0.064713	87.38	8.56	3.78	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237398	M	M	NA	Agree	0.00	2.05	0.00	20.50	4.36	37.15	NONE	POLYA	0.87966	0.033705	0.019813	0.066822	87.34	8.14	4.14	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237399	M	M	NA	Agree	0.00	2.61	0.00	21.86	6.95	43.61	NONE	POLYA	0.890841	0.05722	0.027239	0.024701	88.00	4.54	6.92	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237400	M	M	NA	Agree	0.01	1.98	0.00	13.31	5.60	39.99	NONE	POLYA	0.88924	0.060363	0.026598	0.023799	89.85	4.76	4.97	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237401	M	M	NA	Agree	0.03	2.42	0.00	19.78	6.48	49.40	NONE	POLYA	0.904668	0.051794	0.026257	0.017282	90.78	3.94	4.92	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237402	M	M	NA	Agree	0.00	1.98	0.00	18.27	5.75	37.25	NONE	POLYA	0.887754	0.053319	0.025204	0.033723	88.41	5.19	5.99	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237403	M	M	NA	Agree	0.01	2.71	0.00	21.08	7.47	45.16	NONE	POLYA	0.858664	0.062132	0.027308	0.051896	87.93	6.80	4.92	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237404	M	M	NA	Agree	0.00	2.24	0.00	12.48	5.16	33.06	NONE	POLYA	0.857905	0.066416	0.02818	0.047499	86.79	6.99	5.65	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237405	M	M	NA	Agree	0.02	3.24	0.00	21.23	6.57	43.91	NONE	POLYA	0.858298	0.061372	0.02409	0.05624	88.50	7.23	3.97	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237406	M	M	NA	Agree	0.00	2.01	0.00	19.82	4.44	36.67	NONE	POLYA	0.903826	0.037161	0.020792	0.03822	89.31	5.51	4.70	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237407	M	M	NA	Agree	0.00	2.25	0.00	22.62	5.79	41.00	NONE	POLYA	0.911094	0.046381	0.02411	0.018415	91.59	3.58	4.42	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237408	M	M	NA	Agree	0.01	2.21	0.00	18.31	5.15	50.75	NONE	POLYA	0.875695	0.05433	0.026087	0.043889	88.95	6.46	4.23	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9237409	M	M	NA	Agree	0.01	1.67	0.00	12.94	5.33	41.88	NONE	POLYA	0.890559	0.058378	0.027113	0.023951	88.95	4.51	6.07	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does