Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9168501	M	M	NA	Agree	0.00	7.29	0.00	43.15	8.49	46.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91788	0.054811	0.024556	0.002753	89.90	2.51	7.17	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168502	M	M	NA	Agree	0.00	4.61	0.00	34.17	7.60	39.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912802	0.059985	0.025583	0.00163	91.05	2.28	6.25	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168503	M	M	NA	Agree	0.00	3.90	0.00	34.83	6.65	48.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911065	0.056939	0.024275	0.007721	90.68	2.92	6.00	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168504	M	M	NA	Agree	0.00	5.54	0.00	50.60	10.21	52.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918524	0.05593	0.024099	0.001447	90.08	2.22	7.22	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168505	M	M	NA	Agree	0.00	5.58	0.00	60.92	7.64	45.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913042	0.059602	0.025777	0.001578	90.19	2.55	6.73	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168506	M	M	NA	Agree	0.02	6.86	0.00	53.00	10.13	50.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925884	0.049764	0.0232	0.001152	90.01	2.56	6.66	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168507	M	M	NA	Agree	0.00	3.11	0.00	34.41	8.20	40.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886023	0.076762	0.028611	0.008604	87.25	3.30	8.47	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168508	M	M	NA	Agree	0.06	2.67	0.00	39.53	7.72	42.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904343	0.065719	0.028167	0.001771	87.53	2.64	9.21	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168509	M	M	NA	Agree	0.00	4.86	0.00	68.32	7.41	44.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917431	0.052417	0.02823	0.001922	87.35	3.05	8.72	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168510	M	M	NA	Agree	0.00	6.67	0.00	58.98	9.78	56.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915748	0.055154	0.027588	0.001509	87.19	2.65	9.36	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168511	M	M	NA	Agree	0.00	2.21	0.00	33.65	7.05	45.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89812	0.066492	0.028759	0.006629	87.22	3.30	8.62	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9168512	M	M	NA	Agree	0.00	3.63	0.00	35.65	8.36	37.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886087	0.077409	0.028864	0.00764	86.89	3.16	9.07	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does