Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9087269	M	M	NA	Agree	0.01	7.07	0.00	32.30	7.49	53.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903855	0.043124	0.021214	0.031808	93.33	5.67	0.71	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087270	M	M	NA	Agree	0.00	5.57	0.00	23.96	4.59	47.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935532	0.01923	0.017303	0.027935	93.85	5.13	0.78	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087271	M	M	NA	Agree	0.01	3.41	0.00	15.78	2.96	40.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936825	0.014826	0.015736	0.032613	93.34	5.58	0.87	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087272	M	M	NA	Agree	0.00	6.87	0.00	30.15	6.52	48.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.92666	0.017067	0.018064	0.038208	92.46	6.30	0.97	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087273	M	M	NA	Agree	0.00	6.21	0.00	26.87	6.27	49.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.948449	0.016338	0.017476	0.017737	94.87	4.16	0.73	6	LP2 (2026-09-30)	PASS	NonPolyA_perM 6 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087274	M	M	NA	Agree	0.01	4.74	0.00	21.39	4.16	51.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940324	0.016079	0.01656	0.027037	93.93	5.04	0.81	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087275	M	M	NA	Agree	0.00	4.24	0.00	24.41	5.70	51.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931543	0.018131	0.019779	0.030546	93.21	5.72	0.75	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087276	M	M	NA	Agree	0.00	4.65	0.00	26.93	5.52	51.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924682	0.0184	0.019577	0.037342	92.57	6.27	0.86	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087277	M	M	NA	Agree	0.00	4.97	0.00	25.60	4.73	47.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924005	0.017335	0.019588	0.039071	92.47	6.41	0.84	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087278	M	M	NA	Agree	0.00	4.07	0.00	23.12	5.03	53.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923713	0.017223	0.018925	0.040139	92.45	6.46	0.82	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087279	M	M	NA	Agree	0.00	4.06	0.00	24.38	5.30	50.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93276	0.016868	0.019605	0.030767	93.30	5.62	0.82	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087280	M	M	NA	Agree	0.00	3.90	0.00	23.26	4.71	54.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929978	0.015887	0.019672	0.034463	92.73	5.96	1.04	7	LP2 (2026-09-30)	PASS	NonPolyA_perM 7 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087281	M	M	NA	Agree	0.01	3.68	0.00	14.31	3.82	23.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.91485	0.029437	0.013794	0.041919	92.51	6.01	1.24	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087282	M	M	NA	Agree	0.01	3.56	0.00	12.69	3.68	35.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.922407	0.01965	0.014023	0.04392	91.50	6.56	1.72	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087283	M	M	NA	Agree	0.00	3.92	0.00	14.79	6.19	24.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894949	0.037396	0.014825	0.05283	91.67	7.08	1.03	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087284	M	M	NA	Agree	0.04	3.37	0.00	16.89	6.59	32.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877066	0.04124	0.016604	0.06509	89.98	8.46	1.28	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087285	M	M	NA	Agree	0.04	2.59	0.00	11.94	5.08	18.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894869	0.031054	0.014063	0.060015	90.36	8.13	1.25	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9087286	M	M	NA	Agree	0.04	4.35	0.00	18.73	7.60	25.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890503	0.033629	0.013634	0.062234	90.29	7.71	1.75	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does