Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9083576	M	M	NA	Agree	0.02	5.52	0.00	24.13	10.68	47.28	NONE	POLYA	0.926233	0.042018	0.026982	0.004767	90.24	3.28	6.26	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083577	M	M	NA	Agree	0.00	4.25	0.00	18.66	9.05	36.77	NONE	POLYA	0.917103	0.048895	0.028265	0.005736	85.99	6.32	7.31	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083578	M	M	NA	Agree	0.06	6.18	0.00	21.90	11.79	38.93	NONE	POLYA	0.917491	0.050331	0.028653	0.003526	90.16	3.78	5.85	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083579	M	M	NA	Agree	0.00	2.10	0.00	6.16	2.24	28.20	NONE	POLYA	0.976167	0.014247	0.007282	0.002303	93.90	2.47	3.54	6	LP2 (2026-09-30)	PASS	NonPolyA_perM 6 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083580	M	M	NA	Agree	0.00	9.25	0.00	45.37	10.07	77.04	NONE	POLYA	0.909365	0.052894	0.033388	0.004352	94.12	1.92	3.85	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083581	M	M	NA	Agree	0.00	0.00	0.00	2.89	0.00	13.68	NONE	POLYA	0.966725	0.018186	0.013018	0.00207	91.17	3.67	4.97	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083582	M	M	NA	Agree	0.00	5.34	0.00	50.94	16.37	80.07	NONE	POLYA	0.882047	0.078734	0.036352	0.002867	93.88	1.91	4.07	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083583	M	M	NA	Agree	0.08	4.80	0.00	36.28	9.62	56.73	NONE	POLYA	0.910164	0.056489	0.030002	0.003345	94.47	1.99	3.36	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9083584	M	M	NA	Agree	0.00	1.32	0.00	18.77	3.85	52.61	NONE	POLYA	0.954034	0.02681	0.015269	0.003887	93.36	2.79	3.70	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does