Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9033765	M	M	NA	Agree	0.01	4.51	0.00	21.57	8.44	47.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939455	0.03251	0.025136	0.002898	96.24	1.83	1.75	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033766	M	M	NA	Agree	0.00	5.18	0.00	27.56	7.59	44.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933995	0.040073	0.023664	0.002268	95.80	1.89	2.06	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033767	M	M	NA	Agree	0.01	5.50	0.00	24.12	9.90	54.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940968	0.028975	0.027382	0.002676	95.40	1.94	2.44	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033768	M	M	NA	Agree	0.01	4.21	0.00	24.76	5.56	40.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939923	0.033907	0.020809	0.005361	95.41	2.29	2.07	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033769	M	M	NA	Agree	0.01	3.52	0.00	31.46	5.29	42.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937975	0.036226	0.021263	0.004536	94.59	2.08	3.10	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033770	M	M	NA	Agree	0.05	3.05	0.00	26.80	5.48	39.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.945669	0.03092	0.0198	0.003611	95.64	2.05	2.07	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033771	M	M	NA	Agree	0.02	4.51	0.00	24.84	7.11	42.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939851	0.034127	0.022165	0.003857	95.84	1.88	2.11	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033772	M	M	NA	Agree	0.02	4.75	0.00	25.82	8.86	51.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935882	0.038584	0.022205	0.003329	95.70	1.84	2.30	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9033773	M	M	NA	Agree	0.01	3.33	0.00	21.18	6.97	32.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939024	0.034824	0.021791	0.004361	96.02	1.91	1.85	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does