Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9022974	M	M	NA	Agree	0.00	3.06	0.00	35.05	5.18	40.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937533	0.03789	0.019536	0.005041	87.43	2.61	9.70	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022975	M	M	NA	Agree	0.00	3.59	0.00	44.62	5.64	38.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.941336	0.034377	0.019147	0.00514	88.17	2.67	8.87	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022976	M	M	NA	Agree	0.00	3.42	0.00	44.07	5.73	36.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942295	0.03462	0.018704	0.004381	87.08	2.47	10.09	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022977	M	M	NA	Agree	0.00	3.18	0.00	35.42	5.76	36.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942319	0.035289	0.017297	0.005096	87.88	2.69	9.13	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022978	M	M	NA	Agree	0.00	3.56	0.00	53.31	6.13	43.68	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936943	0.037521	0.021342	0.004194	87.86	2.63	9.12	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022979	M	M	NA	Agree	0.00	2.59	0.00	33.51	4.35	40.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.941872	0.034766	0.019725	0.003637	86.82	2.51	10.19	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022980	M	M	NA	Agree	0.00	2.98	0.00	41.61	6.15	38.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93967	0.036579	0.019371	0.00438	90.25	2.64	6.82	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022981	M	M	NA	Agree	0.00	3.00	0.00	37.10	5.65	37.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939769	0.035805	0.019329	0.005097	88.16	2.79	8.67	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022982	M	M	NA	Agree	0.00	2.84	0.00	33.97	4.33	33.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940777	0.03521	0.018513	0.0055	85.94	2.72	10.97	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022983	M	M	NA	Agree	0.00	2.96	0.00	31.38	6.17	41.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937944	0.037741	0.019529	0.004787	88.94	2.54	8.20	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022984	M	M	NA	Agree	0.00	3.33	0.00	40.95	5.03	35.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.94073	0.036701	0.018452	0.004117	87.80	2.66	9.19	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022985	M	M	NA	Agree	0.00	3.39	0.00	37.05	5.63	38.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936114	0.039928	0.019737	0.004221	87.54	2.41	9.70	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022986	M	M	NA	Agree	0.00	4.77	0.00	52.09	5.55	63.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923689	0.049331	0.023891	0.00309	88.98	2.66	7.97	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022987	M	M	NA	Agree	0.00	3.65	0.00	40.13	5.82	39.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939087	0.036328	0.020503	0.004082	90.00	2.39	7.03	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022988	M	M	NA	Agree	0.00	3.96	0.00	41.89	4.54	46.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937977	0.038261	0.019299	0.004463	89.27	2.91	7.38	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022989	M	M	NA	Agree	0.00	2.34	0.00	33.41	4.55	38.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93616	0.040148	0.018998	0.004694	87.75	2.63	9.28	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022990	M	M	NA	Agree	0.00	3.53	0.00	39.79	5.90	44.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933971	0.042127	0.019697	0.004205	88.66	2.57	8.39	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022991	M	M	NA	Agree	0.02	4.44	0.00	52.23	5.68	58.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927279	0.046577	0.022344	0.003801	88.00	2.67	8.90	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022992	M	M	NA	Agree	0.00	3.41	0.00	31.74	5.21	37.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93968	0.036129	0.02011	0.004081	87.17	2.60	9.71	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022993	M	M	NA	Agree	0.00	3.82	0.00	43.05	5.35	43.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933194	0.042223	0.020352	0.00423	90.08	2.95	6.62	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022994	M	M	NA	Agree	0.00	4.44	0.00	39.59	7.11	44.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.9287	0.046081	0.021059	0.00416	89.28	2.99	7.32	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022995	M	M	NA	Agree	0.00	2.67	0.00	37.07	5.46	38.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942857	0.033581	0.018929	0.004633	89.75	2.61	7.35	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022996	M	M	NA	Agree	0.00	4.79	0.00	53.56	5.52	59.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933012	0.03713	0.026187	0.003671	87.69	2.60	9.19	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9022997	M	M	NA	Agree	0.00	3.96	0.00	35.85	5.75	38.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938634	0.037576	0.018536	0.005254	88.10	2.77	8.64	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does