Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM9021632	M	M	NA	Agree	0.05	4.68	0.00	19.87	8.13	61.68	NONE	POLYA	0.760087	0.086376	0.034683	0.118854	83.80	12.64	3.34	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021633	M	M	NA	Agree	0.00	3.40	0.00	23.70	9.09	65.06	NONE	POLYA	0.78602	0.078365	0.033273	0.102343	86.62	11.29	1.88	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021634	M	M	NA	Agree	0.00	9.22	0.00	29.79	9.95	62.30	NONE	POLYA	0.830646	0.0666	0.036402	0.066351	89.83	8.10	1.82	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021635	M	M	NA	Agree	0.00	5.32	0.00	22.70	7.77	49.22	NONE	POLYA	0.767736	0.076403	0.032767	0.123094	84.57	13.30	1.90	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021636	M	M	NA	Agree	0.07	6.48	0.00	20.49	10.19	50.40	NONE	POLYA	0.802136	0.074198	0.031907	0.091759	86.20	10.28	3.32	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021637	M	M	NA	Agree	0.00	7.24	0.00	15.54	6.97	56.05	NONE	POLYA	0.804331	0.048281	0.032191	0.115198	85.21	12.60	1.97	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021638	M	M	NA	Agree	0.04	8.22	0.00	26.54	13.37	66.35	NONE	POLYA	0.835442	0.075637	0.035421	0.053499	87.08	6.68	6.03	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021639	M	M	NA	Agree	0.01	6.63	0.00	19.30	8.77	56.43	NONE	POLYA	0.899759	0.030248	0.030072	0.039921	90.31	5.26	4.29	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021640	M	M	NA	Agree	0.00	6.49	0.00	14.27	8.35	46.15	NONE	POLYA	0.786648	0.081365	0.030938	0.101048	86.65	11.12	2.08	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021641	M	M	NA	Agree	0.03	6.86	0.00	21.30	11.95	57.83	NONE	POLYA	0.830212	0.08	0.033368	0.056421	89.00	6.89	3.95	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021642	M	M	NA	Agree	0.01	5.29	0.00	20.11	8.62	57.71	NONE	POLYA	0.901271	0.039959	0.0329	0.02587	91.94	4.10	3.75	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021643	M	M	NA	Agree	0.06	5.43	0.00	21.30	11.35	57.08	NONE	POLYA	0.893845	0.051784	0.034321	0.02005	92.48	3.62	3.67	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021644	M	M	NA	Agree	0.00	6.01	0.00	20.50	10.62	57.63	NONE	POLYA	0.898175	0.053455	0.03357	0.0148	93.37	3.16	3.28	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021645	M	M	NA	Agree	0.02	6.10	0.00	18.43	10.31	56.02	NONE	POLYA	0.907068	0.046514	0.032944	0.013473	92.91	3.00	3.90	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021646	M	M	NA	Agree	0.00	5.03	0.00	18.01	10.22	54.08	NONE	POLYA	0.886943	0.054434	0.033203	0.02542	91.08	3.99	4.76	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021647	M	M	NA	Agree	0.00	4.59	0.00	18.49	9.13	48.25	NONE	POLYA	0.915476	0.033219	0.031667	0.019637	91.93	3.47	4.42	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021648	M	M	NA	Agree	0.00	5.03	0.00	17.46	10.90	51.35	NONE	POLYA	0.900929	0.039153	0.031431	0.028488	90.57	4.28	5.00	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021649	M	M	NA	Agree	0.01	4.72	0.00	16.55	10.05	56.40	NONE	POLYA	0.893577	0.043774	0.032412	0.030237	90.84	4.51	4.49	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021650	M	M	NA	Agree	0.00	4.73	0.00	17.84	9.39	50.46	NONE	POLYA	0.902225	0.042472	0.031568	0.023735	91.42	3.80	4.62	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021651	M	M	NA	Agree	0.00	5.51	0.00	20.80	9.48	49.96	NONE	POLYA	0.906674	0.039829	0.031198	0.022299	91.15	3.72	4.97	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021652	M	M	NA	Agree	0.01	5.22	0.00	16.94	8.45	48.59	NONE	POLYA	0.891551	0.03917	0.030051	0.039228	89.67	5.23	4.95	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021653	M	M	NA	Agree	0.01	5.46	0.00	16.69	7.85	53.13	NONE	POLYA	0.891165	0.035344	0.029444	0.044047	89.36	5.77	4.70	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021654	M	M	NA	Agree	0.01	5.67	0.00	18.84	10.55	49.90	NONE	POLYA	0.893602	0.051322	0.031717	0.02336	90.45	3.87	5.50	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021655	M	M	NA	Agree	0.01	4.91	0.00	17.94	9.65	48.00	NONE	POLYA	0.896732	0.051944	0.031804	0.01952	91.45	3.56	4.79	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021656	M	M	NA	Agree	0.01	5.77	0.00	20.29	9.68	46.11	NONE	POLYA	0.89963	0.048564	0.031834	0.019972	91.33	3.47	5.06	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021657	M	M	NA	Agree	0.02	4.54	0.00	17.93	9.08	51.86	NONE	POLYA	0.909266	0.038339	0.030786	0.021609	90.67	3.58	5.60	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021658	M	M	NA	Agree	0.01	5.25	0.00	18.47	9.38	50.40	NONE	POLYA	0.898313	0.041413	0.03067	0.029604	90.32	4.41	5.10	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021659	M	M	NA	Agree	0.00	4.86	0.00	17.89	9.11	55.93	NONE	POLYA	0.882509	0.045534	0.03178	0.040177	89.91	5.39	4.54	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021660	M	M	NA	Agree	0.02	4.25	0.00	16.55	9.73	47.93	NONE	POLYA	0.884086	0.044949	0.03376	0.037205	89.62	5.07	5.13	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021661	M	M	NA	Agree	0.01	5.39	0.00	18.71	9.02	45.94	NONE	POLYA	0.910395	0.036816	0.031281	0.021508	91.03	3.60	5.22	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021662	M	M	NA	Agree	0.16	7.50	0.00	24.74	14.30	80.83	NONE	POLYA	0.763474	0.110349	0.038724	0.087452	86.25	9.64	3.89	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021663	M	M	NA	Agree	0.17	6.32	0.00	20.88	9.95	61.86	NONE	POLYA	0.749754	0.106854	0.035833	0.107559	86.18	11.58	2.01	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021664	M	M	NA	Agree	0.00	8.34	0.00	25.87	7.02	76.57	NONE	POLYA	0.789685	0.083067	0.037372	0.089876	87.69	9.97	2.14	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021665	M	M	NA	Agree	0.12	7.66	0.00	31.78	10.44	80.98	NONE	POLYA	0.783364	0.092695	0.03609	0.087851	86.38	9.65	3.77	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021666	M	M	NA	Agree	0.00	7.07	0.00	32.04	10.40	94.31	NONE	POLYA	0.808836	0.086629	0.038033	0.066502	89.88	7.79	2.12	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021667	M	M	NA	Agree	0.07	7.78	0.00	27.93	13.77	72.85	NONE	POLYA	0.755526	0.100332	0.035027	0.109115	86.09	11.72	1.98	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021668	M	M	NA	Agree	0.26	8.50	0.00	30.89	15.89	79.61	NONE	POLYA	0.749817	0.130092	0.036959	0.083132	86.30	9.15	4.33	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021669	M	M	NA	Agree	0.00	5.41	0.00	12.72	9.06	48.89	NONE	POLYA	0.804757	0.076448	0.039978	0.078817	88.21	9.40	2.08	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021670	M	M	NA	Agree	0.00	4.93	0.00	19.97	12.64	73.21	NONE	POLYA	0.777028	0.112983	0.038494	0.071495	89.32	8.48	1.92	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021671	M	M	NA	Agree	0.04	7.04	0.00	24.93	14.39	86.68	NONE	POLYA	0.871885	0.071143	0.039187	0.017786	91.71	3.32	4.71	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021672	M	M	NA	Agree	0.05	7.26	0.00	22.67	12.29	91.11	NONE	POLYA	0.883174	0.057322	0.036133	0.023371	92.67	3.80	3.29	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021673	M	M	NA	Agree	0.04	6.12	0.00	25.31	13.91	90.28	NONE	POLYA	0.881278	0.061745	0.036845	0.020133	92.83	3.45	3.50	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021674	M	M	NA	Agree	0.03	8.46	0.00	27.97	15.62	102.21	NONE	POLYA	0.892948	0.052957	0.037445	0.016651	93.36	3.07	3.36	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021675	M	M	NA	Agree	0.02	7.20	0.00	21.65	12.68	87.91	NONE	POLYA	0.881609	0.051384	0.036678	0.030329	90.56	4.52	4.65	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021676	M	M	NA	Agree	0.07	5.87	0.00	20.63	10.99	68.86	NONE	POLYA	0.886097	0.054135	0.039024	0.020744	91.38	3.76	4.56	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021677	M	M	NA	Agree	0.00	6.13	0.00	19.09	9.82	53.50	NONE	POLYA	0.891404	0.044897	0.036615	0.027084	90.43	4.07	5.32	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021678	M	M	NA	Agree	0.09	5.91	0.00	19.56	10.57	73.98	NONE	POLYA	0.882995	0.0555	0.038547	0.022958	91.54	3.84	4.34	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021679	M	M	NA	Agree	0.04	5.25	0.00	18.77	10.00	71.60	NONE	POLYA	0.887361	0.055445	0.039675	0.017519	91.31	3.57	4.76	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021680	M	M	NA	Agree	0.01	5.57	0.00	16.24	10.69	70.77	NONE	POLYA	0.897177	0.039301	0.036682	0.026841	90.93	4.19	4.66	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021681	M	M	NA	Agree	0.02	6.58	0.00	21.43	12.30	63.59	NONE	POLYA	0.882109	0.053482	0.037163	0.027246	90.81	4.03	4.94	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021682	M	M	NA	Agree	0.01	5.91	0.00	20.21	10.29	57.03	NONE	POLYA	0.869462	0.061823	0.038625	0.030089	90.09	4.38	5.27	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021683	M	M	NA	Agree	0.00	5.69	0.00	17.79	9.09	63.99	NONE	POLYA	0.877069	0.055814	0.036969	0.030148	90.10	4.58	4.98	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021684	M	M	NA	Agree	0.01	4.48	0.00	19.53	8.64	54.67	NONE	POLYA	0.871429	0.067042	0.042169	0.01936	91.05	3.74	4.87	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021685	M	M	NA	Agree	0.03	5.38	0.00	18.80	8.40	53.85	NONE	POLYA	0.857438	0.072161	0.040651	0.02975	90.55	4.74	4.38	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021686	M	M	NA	Agree	0.03	5.33	0.00	22.21	10.88	68.36	NONE	POLYA	0.883119	0.059306	0.03948	0.018094	90.94	3.51	5.21	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021687	M	M	NA	Agree	0.04	6.26	0.00	22.67	9.68	69.23	NONE	POLYA	0.895539	0.05227	0.038549	0.013641	91.55	3.13	5.01	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021688	M	M	NA	Agree	0.03	5.81	0.00	18.79	10.46	79.40	NONE	POLYA	0.883603	0.050374	0.038393	0.02763	90.40	4.28	5.05	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021689	M	M	NA	Agree	0.03	4.98	0.00	16.53	10.71	76.12	NONE	POLYA	0.886078	0.046117	0.037666	0.030139	90.16	4.71	4.80	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021690	M	M	NA	Agree	0.03	5.38	0.00	16.90	8.70	67.20	NONE	POLYA	0.888033	0.045195	0.038003	0.028769	90.07	4.44	5.19	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM9021691	M	M	NA	Agree	0.02	5.56	0.00	20.29	10.63	66.09	NONE	POLYA	0.896876	0.045544	0.03979	0.01779	91.71	3.23	4.80	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does