Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8981916	M	M	NA	Agree	0.07	7.69	0.00	38.39	20.08	53.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.800327	0.128515	0.061229	0.00993	89.55	6.41	2.49	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981917	M	M	NA	Agree	0.00	6.19	0.00	44.39	16.03	75.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826271	0.104991	0.062953	0.005784	91.30	6.64	0.48	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981918	M	M	NA	Agree	0.01	7.21	0.00	46.14	17.75	73.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.802624	0.127297	0.063513	0.006567	90.33	6.14	2.00	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981919	M	M	NA	Agree	0.00	4.38	0.00	31.05	12.84	50.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830608	0.099734	0.063896	0.005762	90.43	6.74	1.29	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981920	M	M	NA	Agree	0.02	7.01	0.00	40.49	18.74	71.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8207	0.107244	0.063182	0.008874	91.60	6.62	0.28	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981921	M	M	NA	Agree	0.02	6.95	0.00	43.24	21.12	70.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.80628	0.124107	0.064274	0.00534	92.04	6.00	0.41	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981922	M	M	NA	Agree	0.00	6.66	0.00	41.96	15.73	67.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819499	0.109032	0.063492	0.007977	91.48	6.68	0.29	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981923	M	M	NA	Agree	0.02	6.72	0.00	44.33	16.97	60.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.812181	0.118758	0.063492	0.005569	92.07	6.20	0.24	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981924	M	M	NA	Agree	0.01	8.88	0.00	48.92	18.53	79.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.781709	0.146351	0.066811	0.005129	91.99	6.09	0.31	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981925	M	M	NA	Agree	0.03	6.63	0.00	45.33	19.20	72.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.796835	0.133612	0.065248	0.004305	91.69	6.07	0.63	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981926	M	M	NA	Agree	0.03	8.40	0.00	42.34	18.49	78.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.795712	0.133442	0.066607	0.00424	92.18	5.86	0.40	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981927	M	M	NA	Agree	0.03	5.99	0.00	39.44	15.67	61.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.792272	0.13584	0.066492	0.005396	91.51	6.30	0.52	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981928	M	M	NA	Agree	0.02	6.78	0.00	42.14	16.06	67.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819913	0.112501	0.06257	0.005016	91.71	6.35	0.50	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981929	M	M	NA	Agree	0.00	3.21	0.00	24.38	22.13	44.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851153	0.09835	0.048702	0.001796	96.12	1.90	1.71	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981930	M	M	NA	Agree	0.00	6.56	0.00	34.38	26.76	58.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826824	0.118821	0.053694	0.00066	95.35	1.72	2.65	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981931	M	M	NA	Agree	0.00	5.72	0.00	33.71	24.61	64.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846067	0.101376	0.051676	0.000882	95.76	1.79	2.19	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981932	M	M	NA	Agree	0.02	4.54	0.00	30.78	23.22	45.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843392	0.105336	0.049805	0.001468	96.18	1.82	1.74	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981933	M	M	NA	Agree	0.01	4.52	0.00	28.57	24.09	49.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851885	0.097188	0.050165	0.000761	95.61	1.81	2.29	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981934	M	M	NA	Agree	0.00	4.39	0.00	29.23	23.81	54.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.839729	0.106713	0.051889	0.00167	95.76	1.88	2.09	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981935	M	M	NA	Agree	0.00	4.55	0.00	32.37	24.59	53.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846243	0.10041	0.051396	0.001951	95.46	1.91	2.35	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981936	M	M	NA	Agree	0.00	4.19	0.00	30.35	24.90	51.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838378	0.108145	0.051496	0.001981	95.75	1.90	2.10	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981937	M	M	NA	Agree	0.00	5.34	0.00	27.63	26.03	50.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.839713	0.10644	0.052609	0.001237	95.80	1.84	2.08	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981938	M	M	NA	Agree	0.01	7.14	0.00	36.06	29.75	61.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826613	0.117918	0.054603	0.000866	95.42	1.73	2.56	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981939	M	M	NA	Agree	0.01	5.21	0.00	34.80	24.73	57.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840555	0.106198	0.052004	0.001243	95.54	1.83	2.35	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8981940	M	M	NA	Agree	0.01	2.95	0.00	16.41	20.04	46.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850485	0.095899	0.053072	0.000544	95.26	1.98	2.47	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does