Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8974253	M	M	NA	Agree	0.03	8.13	0.00	44.34	17.01	76.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.744418	0.170323	0.051456	0.033803	89.96	5.05	4.66	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974254	M	M	NA	Agree	0.08	3.79	0.00	28.89	11.35	58.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.759781	0.167179	0.055084	0.017956	90.31	4.09	5.22	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974255	M	M	NA	Agree	0.02	6.54	0.00	37.42	18.06	70.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.739028	0.175307	0.050195	0.03547	91.10	5.24	3.32	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974256	M	M	NA	Agree	0.07	8.20	0.00	44.66	16.39	69.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.715839	0.186929	0.051392	0.045839	89.76	6.28	3.62	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974257	M	M	NA	Agree	0.13	6.75	0.00	40.05	14.65	71.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.729041	0.183074	0.05082	0.037065	91.27	5.43	2.99	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974258	M	M	NA	Agree	0.04	6.08	0.00	37.10	15.12	67.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.738562	0.175694	0.050493	0.035252	91.39	5.29	3.01	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974259	F	F	NA	Agree	215.68	0.00	0.00	0.00	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.728136	0.187211	0.051938	0.032714	91.20	4.98	3.50	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974260	F	F	NA	Agree	93.65	0.00	0.00	0.03	0.00	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.761068	0.159779	0.056305	0.022848	91.52	4.10	4.06	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974261	M	M	NA	Agree	0.03	9.49	0.00	41.80	18.03	69.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.729128	0.186083	0.052634	0.032156	87.37	4.78	7.50	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974262	M	M	NA	Agree	0.07	8.14	0.00	39.21	18.91	68.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.711831	0.196834	0.051543	0.039792	88.80	5.73	5.10	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974263	M	M	NA	Agree	0.04	7.96	0.00	45.68	19.80	70.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.725376	0.186531	0.052338	0.035755	89.84	5.40	4.41	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974264	M	M	NA	Agree	0.17	6.03	0.00	41.46	15.65	64.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.730757	0.184072	0.051579	0.033592	90.28	5.23	4.14	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974265	M	M	NA	Agree	0.03	6.88	0.00	43.22	15.27	72.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.742135	0.175268	0.048904	0.033693	91.62	5.18	2.87	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974266	M	M	NA	Agree	0.06	5.56	0.00	38.48	13.31	72.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.730934	0.185133	0.05039	0.033542	89.56	5.39	4.69	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974267	F	F	NA	Agree	206.25	0.00	0.00	0.00	0.13	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.709944	0.209021	0.051966	0.029069	91.01	4.79	3.83	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8974268	F	F	NA	Agree	203.34	0.00	0.00	0.15	0.00	0.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.718877	0.187854	0.047574	0.045695	90.14	6.13	3.38	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does