Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8930547	M	M	NA	Agree	0.00	4.82	0.00	31.68	11.62	25.10	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.547419	0.372959	0.072702	0.006921	86.94	6.72	3.69	3909	LP2 (2026-09-30)	PASS	NonPolyA_perM 3909 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930548	M	M	NA	Agree	0.01	1.18	0.00	7.38	3.11	7.20	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.566065	0.227781	0.163322	0.042832	68.97	21.17	2.19	14079	LP2 (2026-09-30)	PASS	NonPolyA_perM 14079 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930549	M	M	NA	Agree	0.02	3.13	0.00	21.07	8.74	13.47	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.561536	0.345907	0.086845	0.005712	85.74	7.81	2.98	5241	LP2 (2026-09-30)	PASS	NonPolyA_perM 5241 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930550	M	M	NA	Agree	0.54	7.92	0.00	24.45	13.99	20.29	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.484001	0.432678	0.071353	0.011968	83.31	6.94	7.38	7754	LP2 (2026-09-30)	PASS	NonPolyA_perM 7754 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930551	M	M	NA	Agree	0.41	10.11	0.00	29.24	16.82	20.72	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.46744	0.456011	0.069831	0.006718	89.06	5.08	3.89	6435	LP2 (2026-09-30)	PASS	NonPolyA_perM 6435 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930552	M	M	NA	Agree	0.65	10.92	0.00	27.07	14.29	20.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.442411	0.483977	0.069127	0.004484	87.73	5.12	5.16	6131	LP2 (2026-09-30)	PASS	NonPolyA_perM 6131 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930553	M	M	NA	Agree	0.03	3.25	0.00	16.51	7.88	15.78	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.590613	0.30351	0.09288	0.012997	82.78	9.52	3.76	5809	LP2 (2026-09-30)	PASS	NonPolyA_perM 5809 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930554	M	M	NA	Agree	0.00	0.55	0.00	7.34	3.17	7.00	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.587749	0.195472	0.144574	0.072205	67.11	22.20	3.84	15803	LP2 (2026-09-30)	PASS	NonPolyA_perM 15803 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930555	M	M	NA	Agree	0.02	2.00	0.00	10.03	4.42	10.25	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.59627	0.240857	0.13338	0.029492	74.66	15.82	3.37	13071	LP2 (2026-09-30)	PASS	NonPolyA_perM 13071 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930556	M	M	NA	Agree	0.59	8.30	0.00	25.63	15.37	23.05	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.483902	0.432915	0.070938	0.012245	85.64	6.68	5.36	6897	LP2 (2026-09-30)	PASS	NonPolyA_perM 6897 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930557	M	M	NA	Agree	0.94	13.01	0.00	30.81	17.59	26.65	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.429622	0.503334	0.063217	0.003827	85.98	4.04	8.15	5864	LP2 (2026-09-30)	PASS	NonPolyA_perM 5864 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930558	M	M	NA	Agree	0.47	10.16	0.00	29.50	13.63	17.85	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.45058	0.472581	0.068722	0.008117	87.22	5.33	5.31	6959	LP2 (2026-09-30)	PASS	NonPolyA_perM 6959 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930559	M	M	NA	Agree	0.09	2.25	0.00	12.01	3.80	8.52	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.593627	0.259834	0.133771	0.012768	76.00	14.20	3.25	11951	LP2 (2026-09-30)	PASS	NonPolyA_perM 11951 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930560	M	M	NA	Agree	0.01	1.81	0.00	12.27	4.13	11.79	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.615415	0.251152	0.126787	0.006647	78.26	13.08	3.14	9029	LP2 (2026-09-30)	PASS	NonPolyA_perM 9029 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930561	M	M	NA	Agree	0.00	1.05	0.00	6.33	2.43	6.59	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.637887	0.143294	0.195413	0.023407	64.69	22.47	2.68	19137	LP2 (2026-09-30)	PASS	NonPolyA_perM 19137 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930562	M	M	NA	Agree	0.71	10.06	0.00	25.02	14.26	20.82	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.458856	0.46688	0.065652	0.008613	87.73	5.42	4.85	6181	LP2 (2026-09-30)	PASS	NonPolyA_perM 6181 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930563	M	M	NA	Agree	1.07	8.24	0.00	23.95	16.02	20.39	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.440563	0.492349	0.063679	0.003409	84.59	4.62	9.29	4227	LP2 (2026-09-30)	PASS	NonPolyA_perM 4227 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930564	M	M	NA	Agree	0.68	5.87	0.00	20.73	14.14	15.32	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.451946	0.468664	0.072156	0.007234	86.73	5.82	5.12	8516	LP2 (2026-09-30)	PASS	NonPolyA_perM 8516 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not