Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8930498	M	M	NA	Agree	0.23	2.22	0.00	9.03	6.60	6.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.478023	0.26471	0.104211	0.153056	68.14	24.68	1.73	11896	LP2 (2026-09-30)	PASS	NonPolyA_perM 11896 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930499	M	M	NA	Agree	0.21	4.05	0.00	14.78	6.45	10.73	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.506722	0.324928	0.09971	0.068641	78.03	15.70	1.83	9057	LP2 (2026-09-30)	PASS	NonPolyA_perM 9057 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930500	M	M	NA	Agree	0.25	5.36	0.00	15.25	7.97	13.56	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.423599	0.303932	0.086426	0.186042	68.79	25.81	1.84	10569	LP2 (2026-09-30)	PASS	NonPolyA_perM 10569 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930501	M	M	NA	Agree	0.43	6.51	0.00	18.20	8.76	17.40	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.497042	0.3787	0.095271	0.028987	82.98	10.97	2.24	8640	LP2 (2026-09-30)	PASS	NonPolyA_perM 8640 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930502	M	M	NA	Agree	0.34	5.81	0.00	13.75	8.18	13.22	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.484286	0.378002	0.103426	0.034286	81.11	12.95	1.99	10230	LP2 (2026-09-30)	PASS	NonPolyA_perM 10230 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930503	M	M	NA	Agree	0.23	6.35	0.00	17.85	7.78	15.07	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.492132	0.367388	0.091799	0.04868	81.19	13.14	1.75	8305	LP2 (2026-09-30)	PASS	NonPolyA_perM 8305 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930504	M	M	NA	Agree	0.24	4.68	0.09	21.28	6.49	12.83	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.486227	0.378958	0.092977	0.041837	80.71	12.99	2.66	9236	LP2 (2026-09-30)	PASS	NonPolyA_perM 9236 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930505	M	M	NA	Agree	0.16	4.33	0.00	18.58	6.22	13.95	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.508571	0.34143	0.105283	0.044717	79.98	14.00	1.98	8771	LP2 (2026-09-30)	PASS	NonPolyA_perM 8771 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930506	M	M	NA	Agree	0.26	4.12	0.00	14.90	7.34	11.11	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.473982	0.333747	0.099598	0.092673	76.29	17.97	1.75	9417	LP2 (2026-09-30)	PASS	NonPolyA_perM 9417 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930507	M	M	NA	Agree	0.18	4.14	0.00	15.81	6.81	12.14	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.499709	0.349505	0.103829	0.046957	80.05	14.01	1.79	9109	LP2 (2026-09-30)	PASS	NonPolyA_perM 9109 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930508	M	M	NA	Agree	0.18	3.21	0.00	12.36	5.73	8.69	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.482003	0.306018	0.107343	0.104636	74.04	20.28	1.74	9776	LP2 (2026-09-30)	PASS	NonPolyA_perM 9776 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930509	M	M	NA	Agree	0.18	3.61	0.00	12.39	6.69	11.26	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.425432	0.285075	0.090441	0.199052	66.86	27.82	1.59	10185	LP2 (2026-09-30)	PASS	NonPolyA_perM 10185 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930510	M	M	NA	Agree	0.44	6.66	0.00	16.57	9.53	14.37	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.468106	0.378193	0.090409	0.063293	80.44	14.25	1.97	9270	LP2 (2026-09-30)	PASS	NonPolyA_perM 9270 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930511	M	M	NA	Agree	0.37	5.88	0.00	17.94	9.30	15.35	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.469499	0.361648	0.091776	0.077076	79.06	15.69	1.80	9130	LP2 (2026-09-30)	PASS	NonPolyA_perM 9130 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930512	M	M	NA	Agree	0.73	6.89	0.00	19.29	10.31	15.91	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.449671	0.397091	0.086185	0.067053	80.56	14.22	2.08	9226	LP2 (2026-09-30)	PASS	NonPolyA_perM 9226 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930513	M	M	NA	Agree	0.20	3.43	0.00	12.03	6.05	9.58	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.44882	0.306298	0.107639	0.137243	70.91	23.27	1.71	11150	LP2 (2026-09-30)	PASS	NonPolyA_perM 11150 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930514	M	M	NA	Agree	0.57	5.90	0.00	15.16	7.83	12.67	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.460778	0.371737	0.096593	0.070892	78.72	15.84	1.75	9899	LP2 (2026-09-30)	PASS	NonPolyA_perM 9899 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930515	M	M	NA	Agree	0.52	5.69	0.00	15.75	8.47	14.97	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.44891	0.371205	0.092807	0.087078	77.99	16.68	2.12	9447	LP2 (2026-09-30)	PASS	NonPolyA_perM 9447 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930516	M	M	NA	Agree	0.42	5.04	0.00	15.71	7.83	13.66	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.414843	0.345571	0.092594	0.146992	72.11	22.75	1.95	10048	LP2 (2026-09-30)	PASS	NonPolyA_perM 10048 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930517	M	M	NA	Agree	0.33	4.31	0.00	12.94	6.83	11.54	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.438738	0.319402	0.094716	0.147143	71.47	23.09	1.86	9531	LP2 (2026-09-30)	PASS	NonPolyA_perM 9531 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM8930518	M	M	NA	Agree	0.31	4.62	0.00	13.44	6.25	14.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.418541	0.338677	0.094364	0.148418	71.51	23.21	1.86	9775	LP2 (2026-09-30)	PASS	NonPolyA_perM 9775 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not