Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8761636	M	M	NA	Agree	0.00	1.15	0.00	9.99	2.93	25.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970246	0.010952	0.016965	0.001838	96.61	1.74	1.46	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761637	M	M	NA	Agree	0.00	1.45	0.00	14.12	3.00	25.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.967303	0.01291	0.01821	0.001578	96.58	1.71	1.50	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761638	M	M	NA	Agree	0.00	2.21	0.00	15.05	3.50	26.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.966448	0.013877	0.017833	0.001842	96.56	1.83	1.41	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761639	M	M	NA	Agree	0.00	1.02	0.00	10.82	2.67	28.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970995	0.01091	0.016295	0.001801	96.63	1.86	1.41	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761640	M	M	NA	Agree	0.00	0.73	0.00	8.27	2.19	22.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.973642	0.008573	0.015694	0.002091	96.27	1.88	1.73	7	LP2 (2026-09-30)	PASS	NonPolyA_perM 7 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761641	M	M	NA	Agree	0.01	1.67	0.00	14.70	0.39	26.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.968491	0.013716	0.016476	0.001317	96.97	1.65	1.28	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761642	M	M	NA	Agree	0.00	1.97	0.00	15.71	0.72	31.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.969406	0.013417	0.015868	0.001309	96.89	1.70	1.30	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761643	M	M	NA	Agree	0.00	1.87	0.00	13.54	0.45	26.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970088	0.012475	0.016044	0.001394	96.80	1.71	1.39	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761644	M	M	NA	Agree	0.00	1.77	0.00	12.51	0.64	34.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.973139	0.009688	0.015226	0.001948	96.78	1.73	1.39	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8761645	M	M	NA	Agree	0.00	2.17	0.00	13.27	0.71	29.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.973614	0.010135	0.01431	0.001942	96.90	1.69	1.34	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does