Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8685653	F	F	NA	Agree	32.31	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.939879	0.02585	0.029582	0.004689	94.73	2.31	2.82	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8685654	F	F	NA	Agree	70.42	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.924276	0.037928	0.0321	0.005696	94.87	2.26	2.68	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8685655	F	F	NA	Agree	49.93	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.91852	0.036124	0.034731	0.010625	94.63	2.68	2.59	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8685656	F	F	NA	Agree	47.86	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.92227	0.041667	0.030664	0.005399	94.25	2.58	3.03	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8685657	F	F	NA	Agree	65.20	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.919146	0.03848	0.034624	0.007751	94.67	2.45	2.74	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8685658	F	F	NA	Agree	44.29	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.932157	0.031048	0.031455	0.00534	94.66	2.23	2.92	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does