Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8649002	not_specified	M	NA	Conflict	0.00	3.61	0.00	29.58	3.15	41.19	NONE	POLYA	0.872838	0.025695	0.020688	0.080779	86.40	9.80	3.59	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649003	not_specified	M	NA	Conflict	0.00	4.91	0.00	43.63	5.53	58.57	NONE	POLYA	0.92016	0.029889	0.021119	0.028833	92.27	5.04	2.50	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649004	not_specified	M	NA	Conflict	0.00	5.95	0.00	43.96	4.51	56.61	NONE	POLYA	0.901904	0.028585	0.022676	0.046835	90.13	6.80	2.85	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649005	not_specified	M	NA	Conflict	0.00	5.90	0.00	32.21	10.59	61.57	NONE	POLYA	0.873745	0.048602	0.030429	0.047224	89.40	7.14	3.22	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649006	not_specified	M	NA	Conflict	0.01	3.85	0.00	31.19	6.75	56.66	NONE	POLYA	0.868091	0.0519	0.031893	0.048116	90.71	7.38	1.66	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649007	not_specified	M	NA	Conflict	0.00	5.57	0.00	37.37	7.15	61.51	NONE	POLYA	0.875295	0.047323	0.029179	0.048204	88.64	7.09	4.05	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649008	not_specified	M	NA	Conflict	0.02	4.91	0.00	31.21	8.38	59.59	NONE	POLYA	0.876215	0.041006	0.026045	0.056735	88.81	7.82	3.18	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649009	not_specified	M	NA	Conflict	0.02	7.81	0.00	38.87	10.98	76.23	NONE	POLYA	0.880401	0.054763	0.029528	0.035308	91.56	5.89	2.33	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649010	not_specified	M	NA	Conflict	0.00	7.08	0.00	29.88	7.34	56.60	NONE	POLYA	0.864754	0.046352	0.029469	0.059425	88.55	8.04	3.20	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649011	not_specified	M	NA	Conflict	0.00	5.38	0.00	34.04	6.28	49.18	NONE	POLYA	0.861198	0.034951	0.024287	0.079564	87.41	9.71	2.70	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649012	not_specified	M	NA	Conflict	0.00	4.43	0.00	29.38	7.45	50.62	NONE	POLYA	0.879534	0.035742	0.02655	0.058174	89.08	7.97	2.74	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649013	not_specified	M	NA	Conflict	0.00	5.02	0.00	31.78	8.71	50.38	NONE	POLYA	0.855394	0.056546	0.033428	0.054632	89.33	8.01	2.41	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649014	not_specified	M	NA	Conflict	0.00	6.36	0.00	39.35	4.67	59.86	NONE	POLYA	0.932267	0.029862	0.022702	0.015169	92.66	3.74	3.40	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649015	not_specified	M	NA	Conflict	0.03	4.85	0.00	37.95	6.08	59.54	NONE	POLYA	0.937518	0.036474	0.022204	0.003804	94.69	2.62	2.48	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649016	not_specified	M	NA	Conflict	0.02	3.57	0.00	29.61	4.43	44.64	NONE	POLYA	0.942769	0.032819	0.019152	0.005261	92.88	2.66	4.30	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649017	not_specified	M	NA	Conflict	0.00	5.12	0.00	43.89	6.96	71.98	NONE	POLYA	0.905294	0.031489	0.02538	0.037837	90.67	5.98	3.11	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649018	not_specified	M	NA	Conflict	0.00	6.76	0.00	41.06	6.30	57.28	NONE	POLYA	0.916923	0.032093	0.024789	0.026196	91.53	4.93	3.30	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8649019	not_specified	M	NA	Conflict	0.00	4.79	0.00	33.77	4.72	54.58	NONE	POLYA	0.914662	0.024011	0.021991	0.039336	90.98	6.09	2.71	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does