Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8634092	M	M	NA	Agree	0.03	7.19	0.00	48.05	17.06	65.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861336	0.086568	0.045468	0.006628	92.87	2.03	4.92	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634093	M	M	NA	Agree	0.04	6.39	0.00	40.28	15.24	61.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861179	0.086389	0.046657	0.005775	94.08	2.01	3.74	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634094	M	M	NA	Agree	0.00	7.57	0.00	51.19	16.86	73.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860721	0.087433	0.046556	0.005289	95.11	1.96	2.76	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634095	M	M	NA	Agree	0.06	7.77	0.00	49.01	17.80	67.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854069	0.092203	0.047211	0.006517	94.62	2.04	3.18	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634096	M	M	NA	Agree	0.03	7.91	0.00	50.94	17.93	71.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85458	0.092501	0.047236	0.005684	95.01	1.92	2.90	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634097	M	M	NA	Agree	0.03	8.02	0.00	49.26	19.77	66.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843247	0.104545	0.048482	0.003725	94.52	1.82	3.48	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634098	M	M	NA	Agree	0.03	7.92	0.00	47.47	18.38	72.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86199	0.084455	0.047389	0.006166	94.50	2.06	3.28	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634099	M	M	NA	Agree	0.05	8.99	0.00	54.57	17.53	75.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854998	0.092797	0.046876	0.005328	94.65	1.97	3.21	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634100	M	M	NA	Agree	0.03	7.90	0.00	51.01	18.79	71.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860894	0.086473	0.047089	0.005545	94.74	2.02	3.07	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634101	M	M	NA	Agree	0.02	6.38	0.00	42.02	17.05	64.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855971	0.091645	0.047696	0.004688	93.79	1.93	4.12	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634102	M	M	NA	Agree	0.05	8.19	0.00	42.57	24.90	54.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852705	0.100936	0.041588	0.004771	92.17	2.67	4.82	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634103	M	M	NA	Agree	0.05	9.91	0.00	52.83	25.94	58.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85925	0.097007	0.039987	0.003756	93.35	2.47	3.87	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634104	M	M	NA	Agree	0.06	9.81	0.00	52.58	26.15	64.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853893	0.100267	0.041738	0.004102	93.71	2.63	3.30	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634105	M	M	NA	Agree	0.05	7.90	1.07	47.93	21.50	63.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87152	0.080257	0.041157	0.007066	92.87	3.00	3.76	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634106	M	M	NA	Agree	0.09	8.05	0.00	49.00	26.46	57.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848469	0.102271	0.043461	0.005799	92.42	2.80	4.44	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634107	M	M	NA	Agree	0.02	8.59	0.40	53.02	26.99	55.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851108	0.100014	0.044268	0.00461	94.32	2.73	2.59	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634108	M	M	NA	Agree	0.02	7.18	0.00	45.47	21.28	60.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873081	0.077872	0.044191	0.004857	92.64	2.95	4.03	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634109	M	M	NA	Agree	0.05	8.23	0.00	49.86	27.25	50.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858672	0.094372	0.041972	0.004984	92.87	2.67	4.11	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634110	M	M	NA	Agree	0.04	9.94	0.00	51.69	27.83	53.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858124	0.095947	0.041351	0.004578	92.14	2.67	4.80	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8634111	M	M	NA	Agree	0.05	8.74	0.00	49.47	25.81	54.68	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854196	0.098555	0.043732	0.003517	93.53	2.71	3.36	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does