Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8630177	M	M	NA	Agree	0.02	3.68	0.00	31.90	11.96	104.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89305	0.034523	0.05255	0.019878	89.66	6.56	3.25	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630178	M	M	NA	Agree	0.00	3.89	0.00	23.69	10.82	85.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882122	0.03137	0.051426	0.035081	87.81	7.96	3.69	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630179	M	M	NA	Agree	0.00	5.40	0.00	33.65	12.46	87.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895294	0.031643	0.049388	0.023674	89.80	6.47	3.23	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630180	M	M	NA	Agree	0.00	4.38	0.00	25.41	10.86	81.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879994	0.03485	0.04955	0.035606	87.82	7.91	3.73	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630181	M	M	NA	Agree	0.00	5.13	0.00	36.27	13.02	95.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891637	0.035352	0.051373	0.021638	89.77	6.38	3.33	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630182	M	M	NA	Agree	0.00	5.17	0.00	32.18	14.71	100.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888337	0.029752	0.051378	0.030533	89.04	7.20	3.27	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630183	M	M	NA	Agree	0.03	6.20	0.00	40.57	14.81	89.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88795	0.040995	0.049881	0.021174	90.78	5.95	2.76	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630184	M	M	NA	Agree	0.00	5.12	0.00	37.91	12.35	91.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891792	0.035681	0.048405	0.024122	89.02	6.68	3.79	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8630185	M	M	NA	Agree	0.00	3.33	0.00	23.76	10.08	82.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875138	0.030863	0.050923	0.043076	86.69	8.87	3.88	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does