Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8497144	M	M	NA	Agree	0.00	1.52	0.00	5.53	2.41	22.25	NONE	POLYA	0.946904	0.017342	0.02815	0.007605	91.87	6.34	1.60	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8497145	M	M	NA	Agree	0.00	1.61	0.00	5.93	2.83	22.45	NONE	POLYA	0.902003	0.018806	0.027358	0.051833	88.37	10.03	1.41	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8497146	M	M	NA	Agree	0.00	1.40	0.00	5.56	2.51	21.62	NONE	POLYA	0.949962	0.017615	0.027988	0.004435	92.28	5.93	1.58	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8497147	M	M	NA	Agree	0.00	2.49	0.00	9.29	2.73	32.90	NONE	POLYA	0.9378	0.027764	0.029031	0.005405	92.67	5.59	1.55	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8497148	M	M	NA	Agree	0.01	2.21	0.00	9.03	2.50	34.43	NONE	POLYA	0.903726	0.025428	0.027085	0.043761	88.63	9.31	1.86	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8497149	M	M	NA	Agree	0.00	1.95	0.00	9.46	2.43	35.25	NONE	POLYA	0.939701	0.027062	0.02872	0.004517	91.68	6.16	1.94	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does