Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8432458	M	M	NA	Agree	0.00	6.89	0.00	26.64	9.91	58.88	NONE	POLYA	0.925671	0.039417	0.029475	0.005437	89.77	1.83	8.27	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432459	M	M	NA	Agree	0.00	5.80	0.00	25.85	10.80	52.66	NONE	POLYA	0.926597	0.038882	0.02798	0.00654	90.09	1.90	7.89	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432460	M	M	NA	Agree	0.00	6.18	0.00	29.11	8.88	43.66	NONE	POLYA	0.925455	0.041733	0.028738	0.004074	89.23	1.72	8.92	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432461	M	M	NA	Agree	0.02	7.39	0.00	30.92	10.20	54.29	NONE	POLYA	0.922522	0.040987	0.031448	0.005042	90.72	1.88	7.28	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432462	M	M	NA	Agree	0.00	7.19	0.00	35.05	8.93	53.31	NONE	POLYA	0.927697	0.035974	0.030608	0.005721	91.87	1.90	6.09	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432463	M	M	NA	Agree	0.00	6.95	0.00	38.93	10.14	66.22	NONE	POLYA	0.918011	0.042544	0.031892	0.007553	88.64	2.13	9.08	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432464	M	M	NA	Agree	0.00	7.79	0.00	35.63	9.44	54.78	NONE	POLYA	0.916743	0.045492	0.029339	0.008426	88.82	2.10	8.93	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8432465	M	M	NA	Agree	0.00	7.76	0.00	32.74	9.41	59.47	NONE	POLYA	0.916578	0.04411	0.028699	0.010613	90.00	2.29	7.57	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does