Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8425611	M	M	NA	Agree	0.17	9.18	0.00	36.04	22.97	68.48	NONE	POLYA	0.891297	0.0635	0.043392	0.001811	95.29	2.05	2.20	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425612	M	M	NA	Agree	0.08	6.57	0.00	25.92	14.17	62.46	NONE	POLYA	0.923985	0.035872	0.038241	0.001902	90.57	2.01	6.87	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425613	M	M	NA	Agree	0.04	8.26	0.00	32.20	18.20	58.36	NONE	POLYA	0.912941	0.045496	0.039629	0.001934	90.97	1.97	6.58	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425614	M	M	NA	Agree	0.04	7.05	0.49	27.00	14.17	57.81	NONE	POLYA	0.927883	0.03457	0.036018	0.00153	90.68	1.93	6.99	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425615	M	M	NA	Agree	0.00	6.36	1.14	33.36	16.07	59.43	NONE	POLYA	0.913464	0.0442	0.039566	0.00277	91.01	2.07	6.46	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425616	M	M	NA	Agree	0.03	7.25	0.00	34.20	17.68	63.90	NONE	POLYA	0.918671	0.041286	0.038581	0.001462	91.59	2.02	5.92	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425617	M	M	NA	Agree	0.02	7.79	0.23	36.23	20.61	71.62	NONE	POLYA	0.916332	0.042945	0.039081	0.001642	92.82	1.97	4.83	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425618	M	M	NA	Agree	0.12	7.32	0.00	33.39	19.74	70.50	NONE	POLYA	0.916543	0.039815	0.042456	0.001186	93.57	1.99	3.84	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425619	M	M	NA	Agree	0.06	8.88	0.00	37.35	20.81	73.40	NONE	POLYA	0.911002	0.046494	0.04075	0.001753	93.92	2.04	3.46	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425620	M	M	NA	Agree	0.00	6.82	0.00	20.14	19.93	73.32	NONE	POLYA	0.925297	0.028425	0.043235	0.003043	94.25	2.16	3.23	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425621	M	M	NA	Agree	0.03	10.44	0.00	40.35	22.38	77.06	NONE	POLYA	0.902007	0.053752	0.043054	0.001188	95.07	1.87	2.71	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425622	M	M	NA	Agree	0.10	8.74	0.00	38.96	23.26	74.82	NONE	POLYA	0.913308	0.044027	0.041389	0.001276	95.00	1.99	2.45	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425623	M	M	NA	Agree	0.03	6.92	0.00	29.80	16.19	64.01	NONE	POLYA	0.92778	0.032806	0.037794	0.001619	90.18	2.08	7.19	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425624	M	M	NA	Agree	0.04	7.84	0.00	30.75	19.57	57.38	NONE	POLYA	0.924179	0.035587	0.038625	0.001609	90.06	1.93	7.47	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425625	M	M	NA	Agree	0.11	9.06	0.00	33.02	20.01	75.28	NONE	POLYA	0.92566	0.033793	0.039298	0.001249	91.37	1.94	6.37	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425626	M	M	NA	Agree	0.09	5.92	0.00	30.83	16.23	62.55	NONE	POLYA	0.925142	0.034401	0.037988	0.002468	89.44	2.01	8.03	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425627	M	M	NA	Agree	0.08	7.25	0.00	35.27	17.92	68.36	NONE	POLYA	0.920199	0.036352	0.04139	0.00206	92.27	2.03	5.18	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425628	M	M	NA	Agree	0.10	10.62	0.24	35.40	22.16	79.22	NONE	POLYA	0.916652	0.040598	0.041453	0.001297	93.80	2.00	3.66	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425629	M	M	NA	Agree	0.05	9.46	0.00	40.56	25.05	76.90	NONE	POLYA	0.912148	0.04582	0.040818	0.001214	94.94	2.01	2.50	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425630	M	M	NA	Agree	0.04	6.35	0.00	23.71	16.10	67.87	NONE	POLYA	0.924909	0.032292	0.040879	0.00192	91.59	2.03	5.89	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425631	M	M	NA	Agree	0.07	7.18	0.00	30.47	15.97	62.39	NONE	POLYA	0.919874	0.038473	0.039812	0.001841	90.46	1.95	7.16	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425632	M	M	NA	Agree	0.10	7.11	0.00	28.30	16.63	69.71	NONE	POLYA	0.922956	0.037547	0.037588	0.001908	90.92	2.05	6.53	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425633	M	M	NA	Agree	0.06	6.95	0.52	28.05	20.08	68.05	NONE	POLYA	0.916253	0.042781	0.039294	0.001672	90.70	1.97	6.90	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8425634	M	M	NA	Agree	0.04	7.26	0.00	26.03	16.38	61.60	NONE	POLYA	0.927182	0.033228	0.03772	0.00187	90.42	2.03	6.99	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does