Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8402323	M	M	NA	Agree	4.03	5.64	0.00	37.10	9.67	43.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8793	0.079861	0.035509	0.00533	93.96	4.04	1.45	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402324	M	M	NA	Agree	2.65	3.04	0.00	17.93	7.08	26.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903155	0.060066	0.03266	0.004118	93.77	4.49	1.25	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402325	M	M	NA	Agree	4.14	3.86	0.00	23.68	8.86	34.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89735	0.064338	0.034563	0.003749	94.29	3.96	1.18	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402326	M	M	NA	Agree	0.25	1.55	0.00	8.33	4.41	22.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940697	0.023003	0.029131	0.007169	93.27	5.05	1.31	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402327	F	F	NA	Agree	94.66	0.03	0.00	0.25	0.05	0.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879318	0.07879	0.038578	0.003314	94.83	3.48	1.20	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402328	F	F	NA	Agree	76.01	0.02	0.00	0.05	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893732	0.066568	0.035825	0.003874	93.91	4.32	1.22	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402329	F	F	NA	Agree	21.70	0.00	0.00	0.08	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.933706	0.03135	0.028887	0.006058	93.09	5.25	1.27	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402330	F	F	NA	Agree	76.50	0.02	0.00	0.06	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891461	0.066967	0.036545	0.005028	93.89	4.18	1.39	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402331	M	M	NA	Agree	5.61	4.20	0.00	28.91	9.18	35.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884416	0.076574	0.033673	0.005337	94.15	3.94	1.26	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402332	M	M	NA	Agree	2.23	5.22	0.00	28.99	8.17	39.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89052	0.063313	0.03358	0.012586	91.72	6.51	1.16	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402333	M	M	NA	Agree	2.43	5.50	0.00	32.53	9.76	37.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889825	0.072378	0.031572	0.006225	93.70	4.30	1.39	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402334	M	M	NA	Agree	7.07	5.51	0.00	36.64	9.05	46.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882548	0.078619	0.035066	0.003766	93.60	4.40	1.22	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402335	F	F	NA	Agree	94.12	0.09	0.00	0.20	0.02	0.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887119	0.075024	0.033291	0.004566	93.14	4.76	1.44	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402336	F	F	NA	Agree	27.92	0.00	0.00	0.04	0.00	0.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926216	0.040642	0.028327	0.004816	93.88	4.48	1.21	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402337	F	F	NA	Agree	84.13	0.02	0.00	0.09	0.02	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895711	0.06614	0.032943	0.005206	93.81	4.22	1.29	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8402338	F	F	NA	Agree	71.93	0.03	0.00	0.36	0.16	0.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898829	0.062297	0.034416	0.004459	93.66	4.35	1.22	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does