Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8383529	not_specified	F	NA	Conflict	144.13	0.07	0.00	0.07	0.13	0.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87242	0.083655	0.043353	0.000572	89.24	2.12	8.04	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383530	not_specified	M	NA	Conflict	0.65	7.94	0.00	29.98	26.21	64.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868191	0.086762	0.044396	0.000651	89.02	2.11	8.40	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383531	not_specified	M	NA	Conflict	0.24	7.33	0.00	29.77	20.36	61.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87432	0.083409	0.041655	0.000615	89.40	2.02	8.05	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383532	not_specified	M	NA	Conflict	1.51	8.61	0.00	33.15	23.78	60.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864302	0.091869	0.04312	0.000708	90.65	2.02	6.74	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383533	not_specified	M	NA	Conflict	0.32	5.96	0.00	27.26	19.42	54.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877886	0.084303	0.037208	0.000603	90.12	2.00	7.16	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383534	not_specified	M	NA	Conflict	1.16	5.31	0.00	25.55	19.29	51.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880554	0.077986	0.040976	0.000483	89.07	1.96	8.44	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383535	not_specified	F	NA	Conflict	75.55	0.00	0.00	0.13	0.11	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881596	0.070433	0.047213	0.000758	89.34	1.61	8.86	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383536	not_specified	F	NA	Conflict	73.66	0.00	0.00	0.00	0.04	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883843	0.069086	0.046433	0.000638	90.87	1.62	7.33	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383537	not_specified	F	NA	Conflict	74.22	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887489	0.065366	0.046585	0.00056	90.43	1.69	7.70	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383538	not_specified	M	NA	Conflict	0.36	7.82	0.00	27.29	18.20	70.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880521	0.072752	0.046191	0.000536	89.44	1.61	8.77	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383539	not_specified	F	NA	Conflict	80.15	0.00	0.00	0.04	0.05	0.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880908	0.070272	0.047606	0.001214	87.07	1.76	10.97	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8383540	not_specified	F	NA	Conflict	89.93	0.00	0.00	0.05	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879993	0.071906	0.047556	0.000545	90.53	1.64	7.63	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does