Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8353127	M	M	NA	Agree	0.00	3.12	0.00	24.06	12.95	53.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903842	0.022106	0.038993	0.035059	91.74	5.63	2.36	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353128	M	M	NA	Agree	0.00	5.42	0.00	29.43	12.83	77.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902588	0.025284	0.041153	0.030975	92.69	5.47	1.57	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353129	M	M	NA	Agree	0.01	4.56	0.00	27.26	12.68	61.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906611	0.022973	0.039191	0.031225	93.01	5.24	1.52	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353130	M	M	NA	Agree	0.00	4.19	0.00	27.21	13.19	58.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907193	0.02379	0.038358	0.030659	93.25	5.21	1.30	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353131	M	M	NA	Agree	0.00	5.09	0.00	30.01	14.41	67.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900923	0.028814	0.040262	0.030001	92.88	5.01	1.87	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353132	M	M	NA	Agree	0.01	4.66	0.00	27.68	12.58	62.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902115	0.024908	0.039352	0.033626	93.06	5.46	1.24	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353133	M	M	NA	Agree	0.00	4.75	0.00	28.67	12.77	75.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.901792	0.029347	0.044527	0.024334	93.70	4.81	1.21	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353134	M	M	NA	Agree	0.01	5.47	0.00	29.14	12.92	78.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899458	0.029202	0.045844	0.025497	93.31	4.86	1.59	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353135	M	M	NA	Agree	0.00	8.71	0.00	43.10	23.46	129.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902744	0.024049	0.040748	0.032459	93.23	5.47	1.05	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353136	M	M	NA	Agree	0.00	7.62	0.00	40.86	24.95	115.13	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907824	0.024148	0.041119	0.026909	93.40	4.84	1.51	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353137	M	M	NA	Agree	0.00	3.67	0.00	22.27	12.57	61.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.901163	0.024392	0.039781	0.034664	92.65	5.70	1.40	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8353138	M	M	NA	Agree	0.00	3.50	0.00	17.45	8.69	44.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.807057	0.024119	0.036414	0.13241	83.03	14.70	2.01	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does