Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8217480	M	M	NA	Agree	0.03	5.21	0.00	18.63	12.62	63.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884126	0.065128	0.044629	0.006117	57.73	1.70	40.42	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217481	M	M	NA	Agree	0.00	4.58	0.00	18.30	12.35	66.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879638	0.068671	0.043782	0.007909	56.90	1.84	41.11	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217482	M	M	NA	Agree	0.03	6.30	0.00	24.72	14.20	75.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868325	0.061282	0.045941	0.024451	56.98	3.06	39.80	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217483	M	M	NA	Agree	0.00	5.01	0.00	19.82	11.02	68.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868686	0.057786	0.044551	0.028977	57.02	3.38	39.45	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217484	M	M	NA	Agree	0.04	5.66	0.00	27.53	11.80	79.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87718	0.058553	0.04498	0.019287	59.47	2.85	37.52	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217485	M	M	NA	Agree	0.02	6.58	0.00	28.96	11.98	74.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863971	0.072323	0.043746	0.019961	65.45	2.90	31.47	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217486	M	M	NA	Agree	0.00	5.73	0.00	27.17	12.14	63.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876545	0.058117	0.043408	0.02193	64.90	3.09	31.83	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217487	M	M	NA	Agree	0.00	6.87	0.00	24.85	13.94	69.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868322	0.066739	0.043286	0.021653	64.89	3.14	31.79	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217488	M	M	NA	Agree	0.03	6.22	0.00	32.74	15.01	56.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873677	0.062559	0.043859	0.019904	65.91	2.77	31.18	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217489	M	M	NA	Agree	0.02	7.23	0.00	36.43	16.52	59.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878155	0.057588	0.04435	0.019907	64.45	2.69	32.72	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8217490	M	M	NA	Agree	0.03	8.41	0.00	47.14	20.09	73.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873587	0.067572	0.044289	0.014551	65.07	2.17	32.61	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does