Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 22 samples processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8195926	not_specified	M	NA	Conflict	0.00	1.70	0.00	9.06	5.89	36.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.847011	0.023894	0.055922	0.073173	71.80	8.04	19.98	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195927	not_specified	M	NA	Conflict	0.00	1.19	0.00	11.04	5.31	35.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863074	0.023298	0.055691	0.057937	77.11	7.38	15.32	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195928	not_specified	M	NA	Conflict	0.00	1.51	0.00	7.81	6.23	34.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849104	0.025844	0.054107	0.070945	78.07	8.73	13.00	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195929	not_specified	M	NA	Conflict	0.00	1.00	0.00	9.48	5.44	34.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850131	0.024858	0.055501	0.06951	78.84	8.57	12.41	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195930	not_specified	F	NA	Conflict	2.34	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848274	0.028276	0.055428	0.068023	82.29	8.86	8.64	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195931	not_specified	F	NA	Conflict	1.86	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855574	0.026801	0.054402	0.063223	78.39	7.88	13.54	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195932	not_specified	F	NA	Conflict	1.56	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8572	0.025681	0.053392	0.063728	81.25	8.51	10.01	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195933	not_specified	F	NA	Conflict	1.91	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.828777	0.028684	0.056522	0.086017	58.38	7.98	33.45	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195934	not_specified	M	NA	Conflict	0.00	2.38	0.00	10.82	8.85	37.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873549	0.030288	0.054428	0.041735	79.99	5.87	13.96	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195935	not_specified	M	NA	Conflict	0.00	1.72	0.00	9.26	4.63	34.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899703	0.022077	0.055233	0.022987	77.70	4.32	17.82	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195936	not_specified	M	NA	Conflict	0.00	1.44	0.00	10.32	6.80	34.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882745	0.022233	0.054992	0.04003	84.88	6.22	8.72	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195937	not_specified	M	NA	Conflict	0.00	1.26	0.00	7.05	5.27	31.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849996	0.023661	0.053595	0.072748	73.43	8.16	18.24	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195938	not_specified	F	NA	Conflict	1.12	0.03	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861632	0.024962	0.054468	0.058938	82.12	7.76	9.93	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195939	not_specified	F	NA	Conflict	2.78	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.84331	0.028832	0.052097	0.075761	79.34	8.86	11.63	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195940	not_specified	F	NA	Conflict	2.15	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844473	0.027495	0.052448	0.075583	80.23	9.02	10.56	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195941	not_specified	F	NA	Conflict	0.22	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826868	0.01893	0.051652	0.10255	73.98	10.91	14.95	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195942	not_specified	M	NA	Conflict	0.00	1.86	0.00	10.73	6.00	34.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85587	0.029759	0.05459	0.05978	81.05	7.72	11.04	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195943	not_specified	M	NA	Conflict	0.00	1.24	0.00	10.47	5.82	38.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864113	0.027662	0.055339	0.052886	83.23	7.36	9.20	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195944	not_specified	M	NA	Conflict	0.00	1.46	0.00	8.82	5.26	27.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846809	0.021708	0.055029	0.076454	80.70	9.55	9.52	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195945	not_specified	M	NA	Conflict	0.00	0.98	0.00	8.51	5.33	31.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.833963	0.026092	0.052558	0.087386	72.55	9.56	17.71	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195946	not_specified	F	NA	Conflict	1.63	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846674	0.026256	0.05485	0.072219	82.09	9.42	8.28	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195947	not_specified	F	NA	Conflict	1.67	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.81843	0.02438	0.053034	0.104157	76.43	11.76	11.60	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195948	not_specified	F	NA	Conflict	2.08	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.824017	0.027832	0.052099	0.096053	80.29	11.40	8.11	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195949	not_specified	F	NA	Conflict	2.01	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836438	0.027788	0.053693	0.08208	81.52	10.16	8.12	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195950	not_specified	M	NA	Conflict	0.00	1.90	0.00	13.07	6.91	39.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86973	0.032404	0.05919	0.038676	62.33	5.09	32.35	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195951	not_specified	M	NA	Conflict	0.00	0.95	0.00	9.70	4.76	30.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863913	0.02318	0.055402	0.057505	83.17	8.11	8.48	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195952	not_specified	M	NA	Conflict	0.00	1.53	0.00	9.83	3.96	32.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844694	0.024326	0.054688	0.076291	76.03	9.01	14.76	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195953	not_specified	M	NA	Conflict	0.00	1.68	0.00	11.78	6.70	41.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880927	0.023143	0.05563	0.0403	84.21	6.39	9.18	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195954	not_specified	F	NA	Conflict	0.76	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.84111	0.021486	0.054362	0.083042	81.16	10.16	8.47	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195955	not_specified	F	NA	Conflict	1.82	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845929	0.025059	0.052937	0.076075	81.28	9.46	9.06	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195956	not_specified	F	NA	Conflict	0.36	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.196912	0.010187	0.011008	0.781893	19.20	74.85	5.42	311	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 311 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195957	not_specified	F	NA	Conflict	1.42	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.842524	0.024082	0.053352	0.080042	79.11	9.46	11.25	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195958	not_specified	M	NA	Conflict	0.00	1.75	0.00	12.43	6.91	37.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856318	0.028308	0.055964	0.05941	80.93	7.96	10.90	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195959	not_specified	M	NA	Conflict	0.00	1.66	0.00	12.53	5.88	38.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856403	0.026764	0.057131	0.059703	82.86	8.14	8.77	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195960	not_specified	M	NA	Conflict	0.00	1.09	0.00	10.88	5.43	32.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845405	0.023274	0.053174	0.078147	80.33	9.62	9.84	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195961	not_specified	M	NA	Conflict	0.00	1.40	0.00	9.51	5.32	31.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.800929	0.032071	0.050786	0.116214	78.05	12.92	8.83	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195962	not_specified	F	NA	Conflict	1.05	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832579	0.023947	0.053725	0.089748	82.17	10.97	6.67	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195963	not_specified	F	NA	Conflict	1.36	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838488	0.024802	0.054579	0.082131	81.49	10.15	8.17	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195964	not_specified	F	NA	Conflict	2.17	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841447	0.027932	0.053364	0.077258	81.12	9.55	9.13	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195965	not_specified	F	NA	Conflict	2.44	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840569	0.033289	0.054962	0.07118	83.05	9.40	7.32	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195966	not_specified	M	NA	Conflict	0.00	1.34	0.00	8.57	5.48	35.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85051	0.024497	0.054126	0.070866	75.48	8.29	16.04	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195967	not_specified	M	NA	Conflict	0.00	1.09	0.00	9.01	4.97	33.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866563	0.026195	0.054063	0.053178	83.51	7.51	8.79	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195968	not_specified	M	NA	Conflict	0.00	0.72	0.00	8.87	5.44	31.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844652	0.022537	0.054287	0.078524	77.17	9.37	13.26	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195969	not_specified	M	NA	Conflict	0.00	2.08	0.00	11.55	6.56	32.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873476	0.028607	0.054866	0.043051	85.42	6.59	7.81	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195970	not_specified	M	NA	Conflict	0.00	2.08	0.00	11.53	7.36	39.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869254	0.026805	0.05568	0.048262	85.78	6.98	7.06	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195971	not_specified	F	NA	Conflict	2.30	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892156	0.026634	0.055698	0.025512	86.12	4.88	8.81	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195972	not_specified	M	NA	Conflict	0.00	1.83	0.00	11.71	5.54	37.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885058	0.024962	0.055829	0.034152	84.99	5.67	9.13	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8195973	not_specified	F	NA	Conflict	2.10	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841867	0.027126	0.052301	0.078706	77.35	9.13	13.34	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does