Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 16 samples processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8133354	M	M	NA	Agree	0.00	0.01	0.00	0.21	0.14	2.56	NONE	POLYA	0.985823	0.007729	0.006151	0.000296	70.26	2.48	26.71	7	LP2 (2026-09-30)	PASS	NonPolyA_perM 7 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133355	M	M	NA	Agree	0.02	0.04	0.00	0.31	0.34	4.93	NONE	POLYA	0.980624	0.011083	0.007791	0.000502	69.18	2.74	27.76	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133356	M	M	NA	Agree	0.00	0.05	0.00	0.23	0.17	1.74	NONE	POLYA	0.986535	0.006497	0.006597	0.000371	67.68	2.71	28.93	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133357	M	M	NA	Agree	0.00	0.06	0.00	0.25	0.10	3.53	NONE	POLYA	0.985235	0.007243	0.006821	0.000702	70.84	1.90	27.12	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133358	M	M	NA	Agree	0.00	0.00	0.00	0.30	0.21	3.43	NONE	POLYA	0.986086	0.006774	0.006617	0.000523	67.22	2.20	30.47	7	LP2 (2026-09-30)	PASS	NonPolyA_perM 7 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133359	M	M	NA	Agree	0.05	0.03	0.00	0.33	0.29	4.72	NONE	POLYA	0.984604	0.008383	0.006514	0.0005	65.37	2.31	32.16	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133360	M	M	NA	Agree	0.01	0.04	0.00	0.30	0.13	3.64	NONE	POLYA	0.985064	0.008257	0.006453	0.000225	66.03	2.30	31.53	6	LP2 (2026-09-30)	PASS	NonPolyA_perM 6 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133361	M	M	NA	Agree	0.02	0.03	0.00	0.41	0.28	4.50	NONE	POLYA	0.982136	0.009429	0.007309	0.001126	65.97	2.36	31.49	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133362	M	M	NA	Agree	0.00	0.02	0.00	0.17	0.07	2.77	NONE	POLYA	0.986096	0.006577	0.006943	0.000384	68.01	2.63	29.27	6	LP2 (2026-09-30)	PASS	NonPolyA_perM 6 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133363	M	M	NA	Agree	0.01	0.02	0.00	0.23	0.14	3.23	NONE	POLYA	0.985935	0.007596	0.006134	0.000335	66.20	2.43	31.16	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133364	M	M	NA	Agree	0.03	0.05	0.00	0.18	0.26	3.35	NONE	POLYA	0.984354	0.008585	0.006383	0.000678	65.82	2.11	31.98	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133365	M	M	NA	Agree	0.01	0.03	0.00	0.44	0.31	3.88	NONE	POLYA	0.981496	0.01069	0.007228	0.000585	65.76	2.51	31.62	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133366	M	M	NA	Agree	0.01	0.05	0.00	0.26	0.29	3.94	NONE	POLYA	0.983626	0.007943	0.00761	0.000821	69.59	2.47	27.35	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133367	M	M	NA	Agree	0.02	0.02	0.00	0.38	0.15	7.45	NONE	POLYA	0.978793	0.011779	0.008761	0.000667	66.86	2.17	30.35	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133368	M	M	NA	Agree	0.03	0.09	0.00	0.23	0.25	5.91	NONE	POLYA	0.980159	0.010554	0.008843	0.000443	66.34	2.48	30.29	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8133369	M	M	NA	Agree	0.03	0.15	0.00	0.28	0.30	4.95	NONE	POLYA	0.981512	0.009736	0.007808	0.000944	70.44	2.46	26.58	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does