Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8083912	M	M	NA	Agree	0.00	6.01	0.00	33.46	26.17	49.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870266	0.074946	0.041948	0.01284	89.01	4.41	6.11	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083913	M	M	NA	Agree	0.01	5.37	0.00	35.34	22.92	48.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865182	0.077713	0.042692	0.014413	91.50	4.95	2.74	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083914	M	M	NA	Agree	0.06	9.04	0.00	50.62	27.82	59.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867909	0.089315	0.041249	0.001526	93.47	3.40	2.58	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083915	M	M	NA	Agree	0.03	7.49	0.00	48.02	24.46	49.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863078	0.095534	0.039982	0.001405	92.91	3.39	3.08	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083916	M	M	NA	Agree	0.04	9.40	0.00	48.88	31.44	60.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.859234	0.097452	0.0414	0.001913	92.35	3.39	3.68	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083917	M	M	NA	Agree	0.04	9.08	0.00	53.72	31.56	54.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855371	0.1024	0.04119	0.001039	91.57	3.51	4.27	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083918	M	M	NA	Agree	0.04	8.11	0.00	45.03	29.71	55.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877084	0.082546	0.038962	0.001408	91.44	3.70	4.29	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083919	M	M	NA	Agree	0.04	7.64	0.00	43.79	25.00	56.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8904	0.070692	0.037175	0.001733	92.70	3.82	3.03	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083920	M	M	NA	Agree	0.03	7.20	0.00	35.39	21.84	51.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893873	0.070624	0.034463	0.00104	92.75	3.59	3.24	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083921	M	M	NA	Agree	0.01	8.20	0.00	47.70	25.16	62.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882168	0.076041	0.039035	0.002756	91.82	4.55	3.11	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083922	M	M	NA	Agree	0.03	8.73	1.51	53.33	28.49	65.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877823	0.079072	0.039723	0.003382	92.76	3.85	2.93	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083923	M	M	NA	Agree	0.04	6.58	0.95	38.51	23.39	49.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884709	0.077828	0.035982	0.00148	90.76	3.68	5.06	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8083924	M	M	NA	Agree	0.10	13.25	0.00	122.37	15.26	69.43	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.828718	0.131503	0.035341	0.004438	91.60	5.00	2.96	446	LP2 (2026-09-30)	PASS	NonPolyA_perM 446 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM8083925	M	M	NA	Agree	0.13	13.77	0.00	109.32	15.27	64.83	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.829785	0.130769	0.034618	0.004828	91.65	5.09	2.84	407	LP2 (2026-09-30)	PASS	NonPolyA_perM 407 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM8083926	M	M	NA	Agree	0.20	12.98	0.00	99.81	16.07	77.38	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.824269	0.134282	0.036876	0.004573	91.68	5.22	2.59	448	LP2 (2026-09-30)	PASS	NonPolyA_perM 448 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM8083927	M	M	NA	Agree	0.18	13.73	0.00	99.64	18.41	69.22	SECOND_READ_TRANSCRIPTION_STRAND	AMBIGUOUS	0.814994	0.145017	0.035811	0.004178	90.17	5.26	4.09	448	LP2 (2026-09-30)	PASS	NonPolyA_perM 448 is between 350 and 700, outside the range of every calibration study; check the study methods