Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8068877	M	M	NA	Agree	0.07	11.29	0.00	60.51	22.97	101.30	NONE	POLYA	0.876302	0.0664	0.054126	0.003172	87.19	3.21	9.07	173	LP2 (2026-09-30)	PASS	NonPolyA_perM 173 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068878	M	M	NA	Agree	0.08	13.03	0.00	64.90	23.43	111.93	NONE	POLYA	0.86935	0.064263	0.062197	0.00419	86.17	3.46	9.85	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068879	M	M	NA	Agree	0.20	11.77	0.00	53.97	27.82	85.80	NONE	POLYA	0.850465	0.088564	0.057418	0.003552	87.44	3.51	8.45	166	LP2 (2026-09-30)	PASS	NonPolyA_perM 166 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068880	M	M	NA	Agree	0.10	12.43	0.00	66.78	30.88	107.32	NONE	POLYA	0.8574	0.082461	0.052434	0.007704	88.47	3.44	7.61	151	LP2 (2026-09-30)	PASS	NonPolyA_perM 151 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068881	M	M	NA	Agree	0.10	11.73	0.00	52.29	23.33	101.80	NONE	POLYA	0.876527	0.065823	0.050161	0.007489	85.61	3.75	10.15	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068882	M	M	NA	Agree	0.16	13.19	0.00	53.61	24.01	105.03	NONE	POLYA	0.887749	0.057247	0.050102	0.004902	86.43	3.69	9.31	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068883	M	M	NA	Agree	0.19	10.00	0.00	50.08	23.37	80.06	NONE	POLYA	0.862019	0.083945	0.050251	0.003785	87.28	3.21	8.94	174	LP2 (2026-09-30)	PASS	NonPolyA_perM 174 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068884	M	M	NA	Agree	0.11	13.03	0.00	60.21	25.71	99.49	NONE	POLYA	0.865896	0.074722	0.054559	0.004823	87.11	3.48	8.82	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068885	M	M	NA	Agree	0.13	16.44	0.00	79.85	19.15	144.46	NONE	POLYA	0.874782	0.069461	0.050745	0.005012	85.23	3.79	10.49	185	LP2 (2026-09-30)	PASS	NonPolyA_perM 185 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068886	M	M	NA	Agree	0.11	9.19	0.00	49.62	24.15	95.02	NONE	POLYA	0.8817	0.063552	0.049595	0.005153	86.77	3.45	9.27	155	LP2 (2026-09-30)	PASS	NonPolyA_perM 155 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068887	M	M	NA	Agree	0.17	12.68	0.00	62.89	26.27	103.07	NONE	POLYA	0.85683	0.083139	0.054274	0.005757	87.62	3.55	8.27	198	LP2 (2026-09-30)	PASS	NonPolyA_perM 198 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068888	M	M	NA	Agree	0.08	11.58	0.00	68.38	18.42	112.92	NONE	POLYA	0.881472	0.060879	0.051555	0.006095	84.19	3.98	11.26	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068889	M	M	NA	Agree	0.09	12.60	0.00	57.78	23.75	91.60	NONE	POLYA	0.877607	0.063109	0.053089	0.006196	86.24	3.71	9.46	188	LP2 (2026-09-30)	PASS	NonPolyA_perM 188 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068890	M	M	NA	Agree	0.22	10.07	0.00	54.33	23.45	89.87	NONE	POLYA	0.879326	0.06141	0.052727	0.006537	86.55	3.67	9.25	154	LP2 (2026-09-30)	PASS	NonPolyA_perM 154 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068891	M	M	NA	Agree	0.07	13.80	0.00	65.11	23.89	104.30	NONE	POLYA	0.86117	0.080191	0.053513	0.005126	87.19	3.62	8.62	172	LP2 (2026-09-30)	PASS	NonPolyA_perM 172 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068892	M	M	NA	Agree	0.16	14.58	0.00	62.02	24.11	96.73	NONE	POLYA	0.85312	0.087956	0.05144	0.007484	86.90	3.53	9.01	179	LP2 (2026-09-30)	PASS	NonPolyA_perM 179 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8068893	M	M	NA	Agree	0.05	14.63	0.00	68.43	25.83	106.97	NONE	POLYA	0.862421	0.080719	0.051202	0.005658	86.75	3.43	9.29	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does