Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8057915	M	M	NA	Agree	0.00	6.40	0.00	40.15	5.67	59.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.971354	0.014529	0.013613	0.000505	97.49	1.26	1.14	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057916	M	M	NA	Agree	0.01	4.91	0.00	37.91	6.23	54.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.971605	0.014428	0.013495	0.000472	97.37	1.23	1.29	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057917	M	M	NA	Agree	0.23	5.71	0.00	41.85	7.26	52.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970884	0.015423	0.012998	0.000695	97.31	1.17	1.41	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057918	M	M	NA	Agree	1.10	5.44	0.00	29.97	6.16	49.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.97396	0.012629	0.012333	0.001078	97.37	1.17	1.33	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057919	M	M	NA	Agree	0.27	3.16	0.00	26.22	6.03	32.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.969084	0.01684	0.013366	0.000711	97.15	1.30	1.38	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057920	M	M	NA	Agree	0.33	3.58	0.00	24.60	6.07	32.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970496	0.015579	0.013181	0.000744	97.39	1.24	1.21	7	LP2 (2026-09-30)	PASS	NonPolyA_perM 7 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057921	M	M	NA	Agree	0.00	3.69	0.00	26.74	6.30	50.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.977684	0.011693	0.009743	0.00088	97.35	1.01	1.52	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057922	M	M	NA	Agree	0.00	3.35	0.00	25.85	6.82	45.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.974134	0.013485	0.011312	0.001069	96.82	1.16	1.87	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8057923	M	M	NA	Agree	0.00	4.78	0.00	26.29	6.80	50.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.970762	0.015844	0.012514	0.00088	96.55	1.30	1.98	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does