Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM8012328	M	M	NA	Agree	0.65	5.00	0.00	14.66	17.31	105.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.802693	0.139118	0.045769	0.01242	94.09	2.95	2.56	183	LP2 (2026-09-30)	PASS	NonPolyA_perM 183 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012329	M	M	NA	Agree	0.14	4.52	0.00	22.12	17.09	103.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834021	0.10933	0.043961	0.012688	94.54	3.12	1.99	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012330	M	M	NA	Agree	0.24	6.64	0.00	25.24	19.54	95.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826541	0.117912	0.044556	0.01099	94.84	2.80	2.07	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012331	M	M	NA	Agree	0.32	1.86	0.00	5.10	3.46	77.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.83572	0.103098	0.045066	0.016116	93.25	3.67	2.68	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012332	M	M	NA	Agree	0.38	7.13	0.00	28.77	29.19	113.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.809561	0.128347	0.04286	0.019233	93.56	3.48	2.60	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012333	M	M	NA	Agree	0.57	4.02	0.00	13.18	15.49	116.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.790916	0.119203	0.045396	0.044486	90.78	6.20	2.59	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012334	M	M	NA	Agree	0.07	5.85	0.00	24.50	16.12	68.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838534	0.10997	0.045104	0.006393	95.61	2.29	1.85	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012335	M	M	NA	Agree	0.10	2.81	0.00	12.65	8.42	68.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86044	0.086448	0.044954	0.008158	94.96	2.82	1.93	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012336	M	M	NA	Agree	0.09	4.04	0.00	18.71	10.34	81.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85182	0.095394	0.046176	0.006611	95.09	2.74	1.79	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012337	M	M	NA	Agree	0.11	2.38	0.00	7.13	3.79	63.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873499	0.075334	0.041862	0.009305	94.70	2.98	2.00	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012338	M	M	NA	Agree	0.22	0.97	0.00	2.48	1.25	66.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850139	0.096959	0.047148	0.005754	94.15	2.76	2.66	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012339	M	M	NA	Agree	0.60	2.09	0.00	6.42	5.30	95.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.817505	0.126287	0.046148	0.01006	93.76	2.94	2.89	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012340	M	M	NA	Agree	0.37	8.49	0.00	31.41	26.88	140.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821775	0.125604	0.045235	0.007387	95.25	2.24	2.20	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012341	M	M	NA	Agree	0.33	1.56	0.00	4.54	3.29	76.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.835564	0.102426	0.043752	0.018257	93.04	3.82	2.73	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012342	M	M	NA	Agree	0.44	7.98	0.00	35.52	31.77	142.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.799299	0.146915	0.047823	0.005963	95.48	2.04	2.17	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012343	M	M	NA	Agree	0.21	3.51	0.00	10.69	7.04	87.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852315	0.09079	0.043974	0.012921	94.35	3.23	2.09	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM8012344	M	M	NA	Agree	0.50	2.54	0.00	9.49	7.06	113.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.817339	0.12919	0.047774	0.005697	94.19	2.60	2.77	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does