Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7993754	M	M	NA	Agree	0.00	5.36	0.00	41.70	16.45	80.14	NONE	POLYA	0.888436	0.077412	0.034042	0.00011	96.12	1.89	1.80	139	LP2 (2026-09-30)	PASS	NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993755	M	M	NA	Agree	0.00	5.71	0.00	38.99	19.19	89.77	NONE	POLYA	0.875997	0.086096	0.037772	0.000136	95.81	1.84	2.15	143	LP2 (2026-09-30)	PASS	NonPolyA_perM 143 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993756	M	M	NA	Agree	0.01	5.15	0.00	39.05	14.36	82.13	NONE	POLYA	0.889498	0.076106	0.034243	0.000153	95.87	1.90	2.05	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993757	M	M	NA	Agree	0.00	5.71	0.00	55.45	13.42	69.12	NONE	POLYA	0.882446	0.082832	0.034511	0.000211	88.78	1.94	9.07	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993758	M	M	NA	Agree	0.00	4.90	0.00	56.14	10.11	75.13	NONE	POLYA	0.893985	0.073914	0.031947	0.000154	96.68	2.06	1.07	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993759	M	M	NA	Agree	0.00	6.76	0.00	61.75	13.85	76.48	NONE	POLYA	0.876383	0.089322	0.034144	0.000151	96.56	2.06	1.17	175	LP2 (2026-09-30)	PASS	NonPolyA_perM 175 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993760	M	M	NA	Agree	0.00	4.26	0.00	56.61	10.95	87.02	NONE	POLYA	0.867253	0.097421	0.035159	0.000167	96.17	1.83	1.76	174	LP2 (2026-09-30)	PASS	NonPolyA_perM 174 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993761	M	M	NA	Agree	0.00	6.98	0.00	66.09	15.48	92.36	NONE	POLYA	0.883114	0.082161	0.034554	0.000171	96.71	1.94	1.15	174	LP2 (2026-09-30)	PASS	NonPolyA_perM 174 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7993762	M	M	NA	Agree	0.02	8.56	0.00	61.52	15.34	94.69	NONE	POLYA	0.894908	0.071756	0.033191	0.000145	96.76	1.95	1.08	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does