Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7932320	M	M	NA	Agree	0.11	5.05	0.00	93.39	12.12	88.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.805182	0.146091	0.047333	0.001395	95.57	2.81	1.18	229	LP2 (2026-09-30)	PASS	NonPolyA_perM 229 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932321	M	M	NA	Agree	0.02	8.41	0.00	116.78	11.96	74.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851056	0.102509	0.045112	0.001323	96.41	2.13	1.14	206	LP2 (2026-09-30)	PASS	NonPolyA_perM 206 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932322	M	M	NA	Agree	0.08	7.24	0.00	95.70	12.68	89.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.815923	0.133094	0.049327	0.001656	95.50	2.77	1.24	241	LP2 (2026-09-30)	PASS	NonPolyA_perM 241 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932323	M	M	NA	Agree	0.17	10.79	0.00	126.82	14.36	86.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.804412	0.148327	0.046429	0.000833	93.44	2.95	2.90	275	LP2 (2026-09-30)	PASS	NonPolyA_perM 275 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932324	M	M	NA	Agree	0.14	8.45	0.00	99.54	12.89	81.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.811061	0.139753	0.048283	0.000903	94.68	3.28	1.14	291	LP2 (2026-09-30)	PASS	NonPolyA_perM 291 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932325	M	M	NA	Agree	0.21	10.34	0.00	120.13	12.72	94.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.789304	0.153389	0.056272	0.001034	94.27	3.43	1.14	292	LP2 (2026-09-30)	PASS	NonPolyA_perM 292 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932326	M	M	NA	Agree	0.04	8.36	0.00	109.63	11.60	108.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8211	0.129754	0.047157	0.001989	95.62	3.05	0.87	211	LP2 (2026-09-30)	PASS	NonPolyA_perM 211 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932327	M	M	NA	Agree	0.08	6.09	0.00	91.99	12.49	79.30	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.824951	0.128484	0.045715	0.00085	95.85	2.73	1.00	200	LP2 (2026-09-30)	PASS	NonPolyA_perM 200 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932328	M	M	NA	Agree	0.02	8.62	0.00	84.65	13.44	85.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.83501	0.115626	0.048476	0.000888	95.44	2.96	0.99	215	LP2 (2026-09-30)	PASS	NonPolyA_perM 215 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932329	M	M	NA	Agree	0.04	8.13	0.00	128.26	7.70	119.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.774911	0.14843	0.076112	0.000547	93.04	4.06	1.12	207	LP2 (2026-09-30)	PASS	NonPolyA_perM 207 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7932330	M	M	NA	Agree	0.06	8.07	0.00	121.36	7.58	109.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.768545	0.156883	0.073886	0.000687	92.92	4.02	1.31	209	LP2 (2026-09-30)	PASS	NonPolyA_perM 209 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does