Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7905279	M	M	NA	Agree	0.13	19.79	0.00	101.63	22.47	56.55	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.54755	0.368766	0.068923	0.014761	90.07	4.14	3.77	3795	LP2 (2026-09-30)	PASS	NonPolyA_perM 3795 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905280	M	Undetermined	NA	Conflict	116.16	9.73	0.00	44.39	10.92	34.57	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.651365	0.249283	0.079286	0.020067	87.48	6.29	3.44	10679	LP2 (2026-09-30)	PASS	NonPolyA_perM 10679 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905281	M	M	NA	Agree	0.14	18.27	0.00	91.79	24.95	66.10	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.600544	0.312612	0.073249	0.013595	90.29	3.99	3.75	3546	LP2 (2026-09-30)	PASS	NonPolyA_perM 3546 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905282	M	M	NA	Agree	0.21	19.64	0.00	82.30	23.97	60.60	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.613993	0.307097	0.0663	0.01261	90.88	3.87	3.51	3739	LP2 (2026-09-30)	PASS	NonPolyA_perM 3739 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905283	M	M	NA	Agree	0.16	17.68	0.00	79.86	19.72	53.10	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.640669	0.279043	0.068886	0.011403	90.47	4.21	3.32	5809	LP2 (2026-09-30)	PASS	NonPolyA_perM 5809 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905284	M	M	NA	Agree	0.07	9.04	0.00	50.26	11.72	38.17	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.658135	0.265878	0.062681	0.013306	90.29	4.15	3.58	2978	LP2 (2026-09-30)	PASS	NonPolyA_perM 2978 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905285	M	M	NA	Agree	0.10	12.33	0.00	75.04	16.41	60.27	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.71717	0.204561	0.062558	0.015711	89.40	4.62	3.85	3546	LP2 (2026-09-30)	PASS	NonPolyA_perM 3546 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905286	M	M	NA	Agree	0.08	15.81	0.00	79.12	19.60	63.99	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.660343	0.260962	0.064867	0.013827	89.77	4.24	4.19	3506	LP2 (2026-09-30)	PASS	NonPolyA_perM 3506 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905287	M	M	NA	Agree	0.12	18.17	0.00	93.61	25.47	69.52	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.658579	0.253751	0.073975	0.013694	90.17	4.05	3.97	2496	LP2 (2026-09-30)	PASS	NonPolyA_perM 2496 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905288	M	M	NA	Agree	0.13	15.91	0.00	84.63	24.88	59.35	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.66298	0.251805	0.069954	0.015262	90.48	4.27	3.66	3198	LP2 (2026-09-30)	PASS	NonPolyA_perM 3198 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905289	M	M	NA	Agree	0.08	18.98	0.00	90.13	24.60	68.25	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.654565	0.256703	0.068278	0.020453	90.12	4.70	3.52	3430	LP2 (2026-09-30)	PASS	NonPolyA_perM 3430 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905290	M	M	NA	Agree	0.13	19.73	0.00	95.62	22.89	76.56	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.731203	0.190359	0.064514	0.013923	90.83	4.16	3.62	4578	LP2 (2026-09-30)	PASS	NonPolyA_perM 4578 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905291	M	M	NA	Agree	0.13	15.93	0.00	80.45	21.11	60.13	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.653106	0.266325	0.067114	0.013455	90.63	4.21	3.61	4018	LP2 (2026-09-30)	PASS	NonPolyA_perM 4018 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905292	M	M	NA	Agree	0.13	15.92	0.00	73.01	19.85	54.58	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.599755	0.317201	0.068701	0.014343	90.70	4.18	3.57	3872	LP2 (2026-09-30)	PASS	NonPolyA_perM 3872 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905293	M	M	NA	Agree	0.11	15.77	0.00	85.14	19.90	59.37	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.671884	0.241715	0.07396	0.012441	88.76	4.61	3.98	6322	LP2 (2026-09-30)	PASS	NonPolyA_perM 6322 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7905294	M	M	NA	Agree	0.12	17.26	0.00	84.40	22.50	64.05	FIRST_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.680784	0.241658	0.065136	0.012422	91.33	3.98	3.41	3664	LP2 (2026-09-30)	PASS	NonPolyA_perM 3664 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not