Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 3 samples processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7852987	not_specified	M	NA	Conflict	0.14	3.52	0.00	13.44	12.58	42.91	NONE	POLYA	0.940091	0.024264	0.029801	0.005844	93.64	2.22	3.91	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852988	not_specified	M	NA	Conflict	0.04	4.19	0.00	12.21	13.76	48.00	NONE	POLYA	0.942099	0.017181	0.03033	0.01039	92.60	2.76	4.32	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852989	not_specified	M	NA	Conflict	0.02	3.49	0.00	16.28	14.08	45.78	NONE	POLYA	0.941702	0.022266	0.029215	0.006817	94.27	2.31	3.07	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852990	not_specified	M	NA	Conflict	0.01	4.53	0.00	16.85	15.32	41.25	NONE	POLYA	0.942956	0.01983	0.029255	0.007959	94.10	2.43	3.22	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852991	not_specified	M	NA	Conflict	0.01	4.38	0.00	14.25	15.40	57.19	NONE	POLYA	0.938489	0.022536	0.032123	0.006851	93.29	2.48	3.83	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852992	not_specified	M	NA	Conflict	0.00	5.10	0.00	17.51	12.21	55.14	NONE	POLYA	0.925403	0.017839	0.029772	0.026986	91.92	4.31	3.48	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852993	not_specified	M	NA	Conflict	0.00	3.33	0.00	14.48	13.55	40.29	NONE	POLYA	0.947529	0.018963	0.028457	0.005051	93.97	2.15	3.61	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852994	not_specified	M	NA	Conflict	0.02	3.99	0.00	14.92	14.27	43.20	NONE	POLYA	0.936259	0.028119	0.031406	0.004216	90.62	2.27	6.48	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852995	not_specified	M	NA	Conflict	0.03	3.02	0.00	16.38	10.17	46.67	NONE	POLYA	0.94148	0.022754	0.030561	0.005205	93.13	2.26	4.14	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852996	not_specified	M	NA	Conflict	0.11	3.46	0.00	10.97	12.57	49.31	NONE	POLYA	0.947582	0.017588	0.028011	0.006819	92.22	2.37	5.19	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852997	not_specified	M	NA	Conflict	0.65	3.22	0.00	14.05	12.48	57.23	NONE	POLYA	0.948714	0.016654	0.030189	0.004443	92.93	2.29	4.46	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852998	not_specified	M	NA	Conflict	0.01	1.49	0.00	14.33	8.29	44.23	NONE	POLYA	0.95066	0.014731	0.030231	0.004378	93.40	2.28	4.04	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7852999	not_specified	F	NA	Conflict	15.89	0.24	0.00	1.64	0.88	3.88	NONE	POLYA	0.942848	0.022142	0.030296	0.004713	92.10	2.21	5.45	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853000	not_specified	F	NA	Conflict	22.80	0.03	0.00	0.36	0.21	0.31	NONE	POLYA	0.941098	0.019157	0.029103	0.010642	91.60	2.85	5.13	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853001	not_specified	F	NA	Conflict	22.18	0.00	0.00	0.00	0.03	0.00	NONE	POLYA	0.925747	0.02057	0.030351	0.023333	90.37	3.95	5.36	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853002	not_specified	F	NA	Conflict	14.25	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.943909	0.018792	0.029215	0.008084	92.88	2.43	4.50	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853003	not_specified	F	NA	Conflict	18.66	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.942784	0.019731	0.029855	0.00763	92.67	2.65	4.31	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853004	not_specified	F	NA	Conflict	15.46	0.00	0.00	0.05	0.00	0.00	NONE	POLYA	0.95115	0.016606	0.029428	0.002817	92.66	2.14	4.88	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853005	not_specified	F	NA	Conflict	23.78	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.942812	0.019926	0.029321	0.007941	92.82	2.48	4.46	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853006	not_specified	F	NA	Conflict	12.38	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.947375	0.016625	0.02886	0.007139	92.83	2.36	4.61	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853007	not_specified	F	NA	Conflict	16.62	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.947279	0.017598	0.029737	0.005387	93.16	2.29	4.16	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853008	not_specified	F	NA	Conflict	12.76	0.00	0.00	0.19	0.04	0.12	NONE	POLYA	0.94821	0.017272	0.028625	0.005893	93.17	2.24	4.32	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853009	not_specified	F	NA	Conflict	19.31	0.22	0.00	0.95	0.31	1.21	NONE	POLYA	0.946706	0.019344	0.029553	0.004397	92.58	2.21	4.88	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853010	not_specified	F	NA	Conflict	23.17	0.20	0.00	0.55	0.96	1.78	NONE	POLYA	0.945345	0.019022	0.03145	0.004184	92.97	2.12	4.62	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853011	not_specified	M	NA	Conflict	0.02	3.21	0.00	10.33	9.68	37.91	NONE	POLYA	0.942867	0.0178	0.029245	0.010088	92.68	2.64	4.44	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853012	not_specified	M	NA	Conflict	0.76	3.27	0.00	12.50	9.01	39.62	NONE	POLYA	0.941662	0.017346	0.030974	0.010018	87.78	2.42	9.58	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853013	not_specified	M	NA	Conflict	1.10	4.76	0.00	18.48	11.44	58.26	NONE	POLYA	0.923812	0.01801	0.032135	0.026043	89.10	4.01	6.68	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853014	not_specified	M	NA	Conflict	0.00	2.95	0.00	6.60	8.26	36.60	NONE	POLYA	0.942604	0.015062	0.029541	0.012793	91.34	2.92	5.53	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853015	not_specified	M	NA	Conflict	0.00	3.51	0.00	13.61	10.58	43.96	NONE	POLYA	0.921639	0.019356	0.029294	0.029711	91.33	4.49	3.86	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853016	not_specified	M	NA	Conflict	0.00	3.32	0.00	16.38	7.39	43.67	NONE	POLYA	0.811253	0.023495	0.027684	0.137568	82.02	14.55	3.16	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853017	not_specified	M	NA	Conflict	0.04	5.29	0.00	8.79	11.99	44.62	NONE	POLYA	0.927777	0.031737	0.033176	0.00731	92.06	2.86	4.84	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853018	not_specified	M	NA	Conflict	0.29	5.41	0.00	13.66	11.05	48.01	NONE	POLYA	0.933752	0.030039	0.031796	0.004413	93.24	2.30	4.16	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853019	not_specified	M	NA	Conflict	0.54	2.73	0.00	16.57	8.97	45.20	NONE	POLYA	0.934802	0.026494	0.032963	0.00574	93.34	2.48	3.93	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853020	not_specified	F	NA	Conflict	36.13	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.921774	0.042385	0.030898	0.004943	92.17	2.38	5.21	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853021	not_specified	F	NA	Conflict	19.93	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.877273	0.01893	0.030698	0.073099	87.39	8.55	3.78	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853022	not_specified	F	NA	Conflict	8.55	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.847112	0.014117	0.025678	0.113093	83.76	12.06	3.90	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853023	not_specified	F	NA	Conflict	23.34	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.839753	0.021392	0.025592	0.113264	84.06	12.21	3.49	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853024	not_specified	F	NA	Conflict	20.77	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.898372	0.017637	0.029807	0.054185	88.44	6.74	4.43	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853025	not_specified	F	NA	Conflict	13.11	0.00	0.00	0.00	0.27	0.29	NONE	POLYA	0.944659	0.019361	0.030632	0.005348	93.52	2.39	3.87	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853026	not_specified	F	NA	Conflict	22.19	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.805115	0.027555	0.025427	0.141904	80.91	14.90	3.98	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853027	not_specified	F	NA	Conflict	13.38	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.854495	0.016564	0.026944	0.101998	85.74	11.02	2.93	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7853028	not_specified	F	NA	Conflict	6.14	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.820803	0.013093	0.024172	0.141931	81.68	14.70	3.38	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does