Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7848777	M	M	NA	Agree	0.00	2.84	0.00	31.57	7.01	75.35	NONE	POLYA	0.935061	0.030665	0.034182	0.000092	93.79	1.43	4.63	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848778	M	M	NA	Agree	0.00	4.16	0.00	31.68	8.16	82.48	NONE	POLYA	0.932198	0.033121	0.034578	0.000103	93.63	1.46	4.75	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848779	M	M	NA	Agree	0.01	3.16	0.00	29.01	7.50	77.01	NONE	POLYA	0.940313	0.026535	0.033049	0.000103	93.38	1.42	5.05	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848780	M	M	NA	Agree	0.00	3.34	0.00	37.76	7.71	81.33	NONE	POLYA	0.908271	0.054825	0.036852	0.000052	91.56	1.59	6.66	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848781	M	M	NA	Agree	0.00	3.29	0.00	30.55	6.72	77.13	NONE	POLYA	0.934777	0.030871	0.034231	0.000122	93.16	1.40	5.26	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848782	M	M	NA	Agree	0.00	3.04	0.00	31.08	7.14	67.77	NONE	POLYA	0.938318	0.027054	0.034454	0.000174	92.08	1.51	6.23	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848783	M	M	NA	Agree	0.00	3.29	0.00	32.37	7.50	84.34	NONE	POLYA	0.938005	0.028863	0.033023	0.000109	93.29	1.44	5.10	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848784	M	M	NA	Agree	0.00	2.71	0.00	28.63	7.60	70.49	NONE	POLYA	0.937783	0.028791	0.033316	0.000109	92.41	1.48	5.93	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848785	M	M	NA	Agree	0.00	3.24	0.00	31.10	7.53	78.43	NONE	POLYA	0.93817	0.028948	0.03275	0.000132	93.11	1.43	5.29	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848786	M	M	NA	Agree	0.00	3.65	0.00	34.25	7.55	83.60	NONE	POLYA	0.937205	0.028915	0.033768	0.000113	92.47	1.46	5.88	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848787	M	M	NA	Agree	0.00	2.96	0.00	30.65	7.78	71.42	NONE	POLYA	0.938601	0.027668	0.033629	0.000102	93.60	1.47	4.75	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848788	M	M	NA	Agree	0.00	2.54	0.00	31.76	7.05	73.31	NONE	POLYA	0.940427	0.026575	0.032887	0.000112	93.05	1.44	5.32	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848789	M	M	NA	Agree	0.00	3.22	0.00	30.90	8.54	73.35	NONE	POLYA	0.93167	0.033487	0.034737	0.000106	93.58	1.44	4.81	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848790	M	M	NA	Agree	0.00	2.82	0.00	28.81	7.87	71.25	NONE	POLYA	0.940396	0.026759	0.0327	0.000145	93.43	1.42	5.00	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7848791	M	M	NA	Agree	0.00	3.73	0.00	32.14	7.71	81.88	NONE	POLYA	0.939581	0.026746	0.033589	0.000084	93.67	1.39	4.79	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does