Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7805883	M	M	NA	Agree	0.01	5.81	0.00	20.86	7.58	57.86	NONE	POLYA	0.925122	0.037118	0.02971	0.008051	93.02	2.51	4.30	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805884	M	M	NA	Agree	0.03	5.71	0.00	23.33	8.43	57.32	NONE	POLYA	0.932027	0.033334	0.030552	0.004087	95.79	2.06	1.96	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805885	M	M	NA	Agree	0.01	7.55	0.00	28.91	7.91	62.34	NONE	POLYA	0.917817	0.045244	0.031774	0.005165	95.39	2.42	2.02	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805886	M	M	NA	Agree	0.01	5.82	0.00	27.74	8.39	58.03	NONE	POLYA	0.905271	0.051581	0.033783	0.009365	95.21	2.46	2.12	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805887	M	M	NA	Agree	0.04	5.59	0.00	24.66	9.47	59.09	NONE	POLYA	0.9187	0.044707	0.033865	0.002727	95.64	2.10	2.03	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805888	M	M	NA	Agree	0.02	5.81	0.00	25.42	10.39	65.41	NONE	POLYA	0.919499	0.045978	0.031006	0.003517	93.42	2.03	4.38	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805889	M	M	NA	Agree	0.02	6.19	0.00	24.70	9.77	62.70	NONE	POLYA	0.920384	0.04408	0.032703	0.002833	93.53	2.04	4.27	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805890	M	M	NA	Agree	0.00	6.33	0.00	23.41	9.63	58.61	NONE	POLYA	0.922367	0.043275	0.031243	0.003114	93.69	1.98	4.16	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805891	M	M	NA	Agree	0.01	5.94	0.00	23.17	8.94	59.45	NONE	POLYA	0.930143	0.036345	0.030438	0.003073	93.80	1.98	4.06	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7805892	M	M	NA	Agree	0.02	5.08	0.00	19.97	9.60	55.50	NONE	POLYA	0.927588	0.037276	0.030025	0.005112	93.71	2.25	3.89	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does