Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7795932	M	M	NA	Agree	0.00	9.04	0.00	49.84	13.68	58.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.950062	0.021478	0.025261	0.003199	94.49	2.07	3.28	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795933	M	M	NA	Agree	0.00	0.93	0.00	3.00	2.02	18.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.944983	0.018794	0.020564	0.015659	92.95	3.69	3.25	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795934	M	M	NA	Agree	0.01	9.84	0.00	39.12	18.70	71.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898389	0.067965	0.031642	0.002004	93.49	2.04	4.21	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795935	M	M	NA	Agree	0.00	8.78	0.00	42.26	14.73	67.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.947774	0.02201	0.025856	0.00436	94.32	2.22	3.28	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795936	M	M	NA	Agree	0.00	3.27	0.00	14.85	7.24	47.90	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.959337	0.020496	0.015797	0.00437	94.19	1.96	3.74	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795937	M	M	NA	Agree	0.00	9.70	0.00	41.02	15.24	74.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942258	0.023652	0.028892	0.005198	93.41	2.39	4.03	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795938	M	M	NA	Agree	0.00	7.15	0.00	32.37	10.60	58.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.938312	0.030663	0.028513	0.002511	94.52	1.87	3.44	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795939	M	M	NA	Agree	0.00	7.88	0.00	36.44	12.44	49.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.931698	0.034531	0.03131	0.002461	91.90	1.86	6.07	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795940	M	M	NA	Agree	0.00	8.15	0.00	32.37	17.62	54.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862377	0.101131	0.034018	0.002474	93.27	1.95	4.52	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795941	M	M	NA	Agree	0.01	6.74	0.00	26.24	14.79	47.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866041	0.102154	0.030028	0.001777	92.84	1.69	5.13	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795942	M	M	NA	Agree	0.02	6.34	0.00	25.02	10.75	55.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908656	0.045423	0.043983	0.001937	93.45	2.00	4.43	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795943	M	M	NA	Agree	0.00	8.70	0.00	52.46	15.19	59.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.949262	0.022295	0.026973	0.001469	94.15	2.00	3.70	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795944	M	M	NA	Agree	0.00	6.78	0.00	45.06	9.72	58.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.957182	0.017996	0.023962	0.000859	96.41	1.62	1.86	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795945	M	M	NA	Agree	0.00	10.47	0.00	43.83	16.79	63.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913937	0.055938	0.027231	0.002893	93.90	1.96	3.94	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795946	M	M	NA	Agree	0.01	8.29	0.00	48.69	15.90	73.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939257	0.032461	0.026312	0.00197	93.35	2.02	4.48	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795947	M	M	NA	Agree	0.00	5.08	0.00	33.13	13.83	70.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897869	0.069181	0.029543	0.003407	93.81	2.10	3.92	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795948	M	M	NA	Agree	0.00	7.69	0.00	42.33	10.04	86.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.944778	0.024672	0.027367	0.003183	93.65	2.30	3.92	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795949	M	M	NA	Agree	0.01	8.95	0.00	52.69	20.92	55.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856497	0.102097	0.037385	0.004022	94.25	1.95	3.65	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795950	M	M	NA	Agree	0.08	9.32	0.00	26.81	28.92	66.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870614	0.094019	0.030986	0.00438	94.88	2.42	2.46	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795951	M	M	NA	Agree	0.02	7.15	0.00	35.75	12.27	57.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924581	0.040539	0.032143	0.002737	94.71	2.36	2.76	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795952	M	M	NA	Agree	0.02	9.03	0.00	28.36	10.15	52.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.773123	0.03183	0.0271	0.167947	76.93	17.81	5.10	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7795953	M	M	NA	Agree	0.00	8.54	0.00	38.94	11.78	64.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926049	0.033608	0.026493	0.01385	91.90	3.44	4.51	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does