Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7518725	M	M	NA	Agree	0.06	7.43	0.00	29.45	12.43	59.24	NONE	POLYA	0.884928	0.04977	0.030379	0.034922	89.56	5.75	4.43	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7518726	M	M	NA	Agree	0.04	4.72	0.00	22.13	9.03	47.17	NONE	POLYA	0.881001	0.043459	0.028568	0.046972	88.59	6.87	4.32	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7518727	M	M	NA	Agree	0.01	6.54	0.00	23.67	11.16	47.47	NONE	POLYA	0.903069	0.045864	0.028807	0.02226	90.28	4.74	4.74	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7518728	M	M	NA	Agree	0.01	7.77	0.00	25.88	10.17	64.94	NONE	POLYA	0.870068	0.045217	0.030492	0.054223	87.98	7.16	4.59	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7518729	M	M	NA	Agree	0.06	7.77	0.00	33.69	11.34	61.43	NONE	POLYA	0.840301	0.053936	0.029177	0.076586	86.35	9.16	4.26	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7518730	M	M	NA	Agree	0.03	6.12	0.00	24.09	10.77	49.66	NONE	POLYA	0.893065	0.043992	0.030348	0.032594	90.65	5.16	3.98	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does