Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7496350	M	M	NA	Agree	0.09	1.80	0.00	10.68	9.95	56.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890601	0.06129	0.045596	0.002514	96.64	1.69	1.48	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496351	M	M	NA	Agree	0.07	1.20	0.00	10.90	9.78	52.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902796	0.049074	0.044605	0.003524	96.14	1.90	1.79	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496352	M	M	NA	Agree	0.02	2.09	0.00	12.55	5.98	50.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902992	0.048124	0.045775	0.003108	96.13	1.87	1.84	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496353	M	M	NA	Agree	0.02	1.36	0.00	11.57	5.98	49.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906781	0.044904	0.045726	0.00259	96.19	1.93	1.72	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496354	M	M	NA	Agree	0.02	1.33	0.00	9.45	4.88	52.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905848	0.043465	0.046225	0.004462	95.78	2.23	1.82	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496355	M	M	NA	Agree	0.01	2.38	0.00	11.50	9.80	56.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898609	0.052676	0.046293	0.002422	96.28	1.79	1.77	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496356	M	M	NA	Agree	0.01	1.63	0.00	10.86	7.47	52.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903754	0.04804	0.044645	0.003561	96.24	1.90	1.69	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496357	M	M	NA	Agree	0.04	1.30	0.00	7.94	5.86	47.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905375	0.04453	0.045208	0.004887	95.71	2.16	1.97	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496358	M	M	NA	Agree	0.01	2.76	0.00	15.42	10.07	55.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888627	0.059018	0.046249	0.006106	95.73	2.15	1.93	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496359	M	M	NA	Agree	0.12	2.20	0.00	12.22	9.35	57.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888455	0.0592	0.046846	0.005499	95.95	2.06	1.83	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496360	M	M	NA	Agree	0.04	1.72	0.00	12.47	8.73	55.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896514	0.054266	0.046015	0.003204	96.05	1.90	1.88	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496361	M	M	NA	Agree	0.02	1.37	0.00	12.24	9.40	56.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88712	0.061561	0.046646	0.004673	96.21	1.94	1.69	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496362	M	M	NA	Agree	0.00	1.61	0.00	12.47	7.13	51.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884135	0.057926	0.044887	0.013051	95.03	2.77	2.04	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496363	M	M	NA	Agree	0.18	1.57	0.00	14.44	9.87	63.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882707	0.063062	0.046926	0.007305	95.75	2.10	1.98	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496364	M	M	NA	Agree	0.06	1.45	0.00	11.88	8.54	56.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897487	0.052241	0.046557	0.003715	96.21	1.97	1.67	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496365	M	M	NA	Agree	0.00	1.47	0.00	12.10	6.46	59.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898746	0.051969	0.045486	0.0038	96.13	1.87	1.84	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496366	M	M	NA	Agree	0.04	2.51	0.00	12.68	10.24	56.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889357	0.060301	0.046818	0.003524	96.36	1.89	1.59	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496367	M	M	NA	Agree	0.07	1.32	0.00	12.49	7.91	56.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.897028	0.053837	0.045742	0.003393	96.33	1.87	1.64	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496368	M	M	NA	Agree	0.01	2.28	0.00	8.73	6.86	52.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895688	0.053038	0.046207	0.005067	94.58	2.12	3.13	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496369	M	M	NA	Agree	0.02	1.19	0.00	6.78	5.50	46.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902612	0.048844	0.04491	0.003634	95.59	2.00	2.25	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496370	M	M	NA	Agree	0.06	2.35	0.00	15.18	10.47	56.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884278	0.063418	0.046822	0.005481	95.92	2.00	1.92	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496371	M	M	NA	Agree	0.04	1.81	0.00	11.99	8.87	52.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887908	0.059862	0.046977	0.005253	95.82	2.02	1.99	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496372	M	M	NA	Agree	0.04	1.76	0.00	9.85	7.57	49.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892638	0.057416	0.04645	0.003496	96.14	1.84	1.86	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496373	M	M	NA	Agree	0.04	1.91	0.00	13.09	10.98	52.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883163	0.064133	0.047553	0.00515	95.93	1.98	1.91	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496374	M	M	NA	Agree	0.14	2.55	0.00	15.88	9.59	57.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87966	0.067931	0.048288	0.004121	95.92	1.84	2.09	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496375	M	M	NA	Agree	0.04	2.33	0.00	9.87	9.77	51.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886625	0.063085	0.046382	0.003908	95.67	1.85	2.31	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496376	M	M	NA	Agree	0.00	3.10	0.00	14.93	13.96	43.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.806484	0.094804	0.046064	0.052649	88.74	6.33	4.69	169	LP2 (2026-09-30)	PASS	NonPolyA_perM 169 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7496377	M	M	NA	Agree	0.06	2.06	0.00	9.27	8.60	46.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892079	0.051356	0.043781	0.012784	95.26	2.78	1.79	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does