Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7449779	M	M	NA	Agree	0.01	10.44	0.00	39.21	21.74	49.88	NONE	POLYA	0.887543	0.067793	0.039745	0.004919	93.35	2.23	4.07	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449780	M	M	NA	Agree	0.04	8.72	0.00	37.00	19.34	51.09	NONE	POLYA	0.895573	0.059928	0.038691	0.005808	93.34	2.32	4.02	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449781	M	M	NA	Agree	0.01	6.83	0.00	34.70	18.16	49.03	NONE	POLYA	0.89332	0.061399	0.039552	0.005729	93.88	2.36	3.37	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449782	M	M	NA	Agree	0.02	8.09	0.44	36.71	18.13	48.05	NONE	POLYA	0.890227	0.062757	0.039582	0.007435	93.33	2.56	3.74	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449783	M	M	NA	Agree	0.03	8.58	0.00	40.02	20.36	55.97	NONE	POLYA	0.881833	0.073204	0.040635	0.004328	93.41	2.25	3.93	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449784	M	M	NA	Agree	0.03	7.67	0.00	40.31	19.88	50.68	NONE	POLYA	0.874694	0.079049	0.041525	0.004732	92.89	2.30	4.39	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449785	M	M	NA	Agree	0.06	5.88	0.00	29.08	20.00	39.89	NONE	POLYA	0.881824	0.06673	0.041945	0.009501	91.91	3.02	4.57	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449786	M	M	NA	Agree	0.06	7.13	0.00	35.44	20.41	42.26	NONE	POLYA	0.869106	0.08039	0.042421	0.008083	92.19	2.74	4.63	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449787	M	M	NA	Agree	0.00	8.45	0.00	39.56	22.55	47.27	NONE	POLYA	0.861757	0.086033	0.042958	0.009251	92.47	2.82	4.30	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449788	M	M	NA	Agree	0.02	8.49	0.00	33.83	16.88	50.02	NONE	POLYA	0.89565	0.061596	0.037346	0.005408	93.04	2.23	4.37	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449789	M	M	NA	Agree	0.02	7.32	0.00	35.86	16.78	51.48	NONE	POLYA	0.892622	0.066766	0.03533	0.005282	93.01	2.21	4.42	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449790	M	M	NA	Agree	0.01	7.44	0.00	33.63	18.63	43.85	NONE	POLYA	0.881215	0.074766	0.03784	0.006179	92.92	2.35	4.37	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449791	M	M	NA	Agree	0.02	7.49	0.00	32.46	16.93	50.98	NONE	POLYA	0.890905	0.067519	0.035886	0.00569	93.54	2.28	3.86	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449792	M	M	NA	Agree	0.05	6.91	0.00	29.37	12.18	55.43	NONE	POLYA	0.914074	0.04793	0.032649	0.005347	93.83	2.32	3.50	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449794	M	M	NA	Agree	0.03	5.99	0.00	31.22	13.88	46.88	NONE	POLYA	0.898268	0.061749	0.035511	0.004472	93.48	2.18	3.96	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449795	M	M	NA	Agree	0.48	6.69	0.00	30.81	15.76	53.16	NONE	POLYA	0.887728	0.068479	0.037975	0.005818	93.55	2.41	3.62	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449796	M	M	NA	Agree	0.49	7.90	0.00	29.83	15.87	49.13	NONE	POLYA	0.870971	0.081051	0.037463	0.010516	92.67	2.81	4.15	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7449797	M	M	NA	Agree	0.32	6.18	0.00	29.17	16.91	45.55	NONE	POLYA	0.880791	0.07627	0.036175	0.006764	93.79	2.45	3.39	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does