Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7119830	M	M	NA	Agree	0.00	2.82	0.00	20.43	5.35	40.21	NONE	POLYA	0.931888	0.03787	0.023719	0.006523	79.87	2.69	16.83	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119831	M	M	NA	Agree	0.02	2.89	0.00	30.76	4.72	49.05	NONE	POLYA	0.942892	0.026734	0.022002	0.008373	80.07	2.80	16.63	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119832	M	M	NA	Agree	0.02	3.02	0.00	26.33	3.26	40.53	NONE	POLYA	0.941234	0.02993	0.022506	0.00633	80.65	2.82	15.88	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119833	M	M	NA	Agree	0.04	3.11	0.00	28.52	4.33	44.77	NONE	POLYA	0.945582	0.025492	0.021846	0.00708	80.35	2.65	16.49	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119834	M	M	NA	Agree	0.02	2.99	0.00	24.31	5.17	44.70	NONE	POLYA	0.943787	0.027295	0.021464	0.007454	80.46	2.72	16.32	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119835	M	M	NA	Agree	0.07	2.46	0.00	26.60	4.13	44.87	NONE	POLYA	0.942833	0.027465	0.020881	0.00882	81.22	2.85	15.39	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119836	M	M	NA	Agree	0.00	2.34	0.00	20.17	4.23	30.26	NONE	POLYA	0.9374	0.035883	0.021716	0.005	80.47	2.54	16.61	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119837	M	M	NA	Agree	0.00	3.22	0.00	23.05	4.56	36.40	NONE	POLYA	0.936736	0.0363	0.021666	0.005298	81.79	2.50	15.32	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119838	M	M	NA	Agree	0.01	4.36	0.00	24.69	5.03	44.66	NONE	POLYA	0.920606	0.040953	0.024663	0.013778	74.60	3.34	21.60	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119839	M	M	NA	Agree	0.03	3.49	0.00	25.14	4.59	39.60	NONE	POLYA	0.935545	0.027379	0.022751	0.014324	76.72	2.94	19.99	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119840	M	M	NA	Agree	0.03	3.96	0.00	24.57	4.36	45.18	NONE	POLYA	0.931982	0.032361	0.025601	0.010056	75.52	3.03	21.12	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119841	M	M	NA	Agree	0.00	3.11	0.00	22.45	3.66	39.01	NONE	POLYA	0.942753	0.024589	0.022869	0.009789	77.35	2.53	19.89	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119842	M	M	NA	Agree	0.02	3.39	0.00	21.13	4.11	41.70	NONE	POLYA	0.935075	0.033168	0.022778	0.008979	76.12	2.79	20.78	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119843	M	M	NA	Agree	0.00	3.72	0.00	25.58	4.58	43.65	NONE	POLYA	0.946162	0.023111	0.021516	0.009211	76.13	2.57	21.04	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119844	M	M	NA	Agree	0.01	3.02	0.00	24.03	3.62	38.81	NONE	POLYA	0.937663	0.029735	0.023283	0.009318	73.89	2.74	22.95	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119845	M	M	NA	Agree	0.06	2.89	0.00	25.23	3.63	37.15	NONE	POLYA	0.931379	0.027402	0.021403	0.019816	77.16	3.51	18.99	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does