Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). For 7 samples processed before workflow 2.1, ComputedSex is the earlier curated call, kept after review where it differed from the rule above.
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7119346	not_specified	F	NA	Conflict	16.79	0.00	0.00	0.00	0.00	0.11	NONE	POLYA	0.945256	0.024496	0.029323	0.000924	90.13	2.51	7.21	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119347	not_specified	F	NA	Conflict	8.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.961363	0.01783	0.019618	0.00119	89.99	3.21	6.69	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119348	not_specified	F	NA	Conflict	8.52	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.957283	0.017185	0.021714	0.003818	92.14	2.06	5.70	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119349	not_specified	F	NA	Conflict	22.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.948869	0.020555	0.027337	0.003239	88.97	2.00	8.89	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119350	not_specified	F	NA	Conflict	13.17	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.950067	0.020671	0.025764	0.003498	89.52	2.45	7.89	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119351	not_specified	F	NA	Conflict	16.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.951713	0.021379	0.024647	0.00226	93.94	2.72	3.21	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119352	not_specified	F	NA	Conflict	12.78	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.955107	0.020574	0.022751	0.001568	93.37	2.31	4.21	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119353	not_specified	F	NA	Conflict	14.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.950295	0.022552	0.024933	0.00222	94.23	2.23	3.44	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119354	not_specified	F	NA	Conflict	13.63	0.00	0.00	0.00	0.00	0.13	NONE	POLYA	0.946704	0.022992	0.024719	0.005584	93.29	2.87	3.74	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119355	not_specified	F	NA	Conflict	15.67	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.943327	0.025636	0.026805	0.004233	93.13	2.92	3.83	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119356	not_specified	F	NA	Conflict	7.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.954357	0.020157	0.019545	0.005941	90.11	5.95	3.81	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119357	not_specified	F	NA	Conflict	19.92	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.956809	0.020831	0.020586	0.001775	94.04	2.06	3.80	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119358	not_specified	F	NA	Conflict	4.49	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.955981	0.017959	0.022595	0.003464	92.82	2.46	4.62	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119359	not_specified	F	NA	Conflict	12.89	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.9553	0.018958	0.022549	0.003193	94.13	2.32	3.44	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119360	not_specified	F	NA	Conflict	15.51	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.955703	0.020371	0.021224	0.002703	94.71	2.09	3.11	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119361	not_specified	F	NA	Conflict	5.88	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.949769	0.019002	0.02677	0.004459	92.52	2.88	4.47	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119362	not_specified	F	NA	Conflict	4.66	0.00	0.00	0.01	0.00	0.04	NONE	POLYA	0.954903	0.018997	0.022353	0.003748	93.08	3.63	3.19	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7119363	not_specified	F	NA	Conflict	5.75	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.96333	0.015708	0.019307	0.001656	94.62	2.00	3.28	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does